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Novel quencher and reporter dye combinations

A technology of quencher and dye, applied in the field of FRET

Pending Publication Date: 2020-10-20
LIFE TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Until now, most multiplex PCR instruments could only utilize four unique dye-quencher pairs

Method used

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  • Novel quencher and reporter dye combinations
  • Novel quencher and reporter dye combinations
  • Novel quencher and reporter dye combinations

Examples

Experimental program
Comparison scheme
Effect test

example

[0202] A quencher compound can be attached to a solid support (e.g., a bead) to provide a substrate for probe construction using an oligonucleotide synthesizer according to the following reaction scheme:

[0203]

[0204] The following exemplary synthetic procedures can be easily generalized to any of the quenchers mentioned above. Accordingly, the reaction schemes above and the procedures below are not intended to limit the scope of the claimed subject matter.

[0205] In one embodiment, a representative derivatized quencher (2) can be synthesized according to the following procedure.

[0206]

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PUM

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Abstract

Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

Description

technical field [0001] The present specification generally relates to FRET pairs comprising quencher and reporter dye combinations for biological applications including, for example, polymerase chain reaction (PCR), such as real-time or quantitative PCR (qPCR). Background technique [0002] Fluorescent energy transfer (FRET) in double-labeled oligonucleotide probes is widely used in the determination of gene analysis. FRET has been used to study DNA hybridization and amplification, protein folding kinetics, proteolytic degradation, and interactions between other biomolecules. Nucleic acid detection / amplification methods such as the use of dual-labeled probes in real-time polymerase chain reaction to detect and / or quantify target nucleic acids such as specific gene sequences or expressed messenger RNA sequences. Fluorescent probes for use in this method are typically labeled with reporter and quencher moieties. In this case, when the two dyes are physically separated by hyb...

Claims

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Application Information

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IPC IPC(8): C07H21/00C12Q1/6818C12Q1/686
CPCC12Q1/6818C12Q1/686C12Q2531/113C12Q2535/122C12Q2537/143C12Q2565/1015C09B11/24C12Q2565/101C12Q2600/156
Inventor K·B·姆拉赫B·伊梵斯S·C·本森张竹安严雄伟
Owner LIFE TECH CORP
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