Novel quencher and reporter dye combinations
A technology of quencher and dye, applied in the field of FRET
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[0202] A quencher compound can be attached to a solid support (e.g., a bead) to provide a substrate for probe construction using an oligonucleotide synthesizer according to the following reaction scheme:
[0203]
[0204] The following exemplary synthetic procedures can be easily generalized to any of the quenchers mentioned above. Accordingly, the reaction schemes above and the procedures below are not intended to limit the scope of the claimed subject matter.
[0205] In one embodiment, a representative derivatized quencher (2) can be synthesized according to the following procedure.
[0206]
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