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Primer group for detecting prawn iridovirus and kit containing primer group

A technology for detecting kits and iridescent viruses, applied in the direction of microorganisms, recombinant DNA technology, and methods based on microorganisms, which can solve the problems of complex detection operations and dependence on large and expensive instruments

Active Publication Date: 2020-10-16
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For the problems existing in the prior art, the purpose of the present invention is to provide a kind of primer group that detects the shrimp iridescent virus and the test kit that contains this primer group, detect the shrimp iridescent virus with the primer group of the present invention, not only overcome the problem in the prior art Relying on large expensive instruments and complicated detection operations; moreover, the primer set of the present invention has high detection sensitivity and good specificity, and is suitable for rapid determination in rough conditions such as aquaculture farms

Method used

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  • Primer group for detecting prawn iridovirus and kit containing primer group
  • Primer group for detecting prawn iridovirus and kit containing primer group
  • Primer group for detecting prawn iridovirus and kit containing primer group

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Embodiment 1

[0048] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0049] The present invention will be described in further detail below in conjunction with specific examples and with reference to data. The following examples are just to illustrate the present invention, but not to limit the scope of the present invention in any way.

[0050] 1. Design and synthesis of primers

[0051] According to the sequence SEQ ID No: 1, LAMP primers were designed using PrimerExplorer V4 software. The specific design principles used were: the Tm value of the inner primer FIP / BIP was selected at about 65°C, the temperature of the outer primer F3 / B3 was about 60°C, and the temperature of the FIP / BIP was about 60°C. The dataG value of the 5' end of BIP is less than or equal to -4kcal / mol, the dataG value of the 3' end of F3 / B3 is less than or equal to -4kcal / mol, the GC content is between 40% and 60%, an...

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Abstract

The invention discloses a primer group for detecting prawn iridovirus and a kit containing the primer group, and belongs to the technical field of aquatic pathogenic organism detection reagents. The primer group for detecting the prawn iridovirus is designed according to a nucleic acid sequence shown in SEQ ID No: 1. The primer group disclosed by the invention is high in sensitivity and good in specificity; the primer group for detecting the prawn iridovirus solves the problems of dependence on large expensive instruments and complex detection operation in the prior art; and moreover, the primer group is suitable for rapid determination of simple and crude conditions such as aquaculture farms.

Description

technical field [0001] The invention belongs to the technical field of detection reagents for aquatic pathogens, and in particular relates to a primer set for detecting prawn iridescent virus and a kit containing the primer set. Background technique [0002] Shrimp hemocyte iridescent virus (SHIV) was found in Penaeus vannamei, which was seriously dead in Zhejiang in 2014. The symptoms of SHIV infection are discoloration, rupture of hepatopancreas, jejunum empty stomach, soft shell, etc.; The histopathological observation of post-virus shrimp showed that the epithelial cells of sick shrimp (gills, stomach lining and even all over the whole body) showed typical infection symptoms of iridescent virus; hematopoietic tissue, antennal gland epithelial tissue, heart, muscle, subcutaneous tissue, gill , connective tissue cells and phagocytes in the hepatopancreas are also infected by iridoviruses. At present, the source of infection and the route of transmission of the disease are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11C12R1/93
CPCC12Q1/701C12Q1/6844C12Q2531/119C12Q2545/113
Inventor 葛辉周宸林琪吴建绍
Owner FISHERIES RES INST OF FUJIAN
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