Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of lectin probe combination in identification of rat sex based on urine protein glycotype

A technology of lectin and urine protein, which is applied in the direction of general structure detail detection of gas analyzers, material inspection products, measuring devices, etc., can solve the problems of not getting enough complete and clear sugar chain distribution information, and achieve low cost Effect

Pending Publication Date: 2020-10-09
NORTHWEST UNIV(CN)
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that the sugar chains in human serum, saliva, and urine are different between men and women, but there is still not enough complete and clear sugar chain distribution information

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of lectin probe combination in identification of rat sex based on urine protein glycotype
  • Application of lectin probe combination in identification of rat sex based on urine protein glycotype
  • Application of lectin probe combination in identification of rat sex based on urine protein glycotype

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The relevant experiments and analysis of the present application are described in detail below, and the inventor's specific research and development process is not limited thereto.

[0023] 1. Experimental part

[0024] 1.1 Reagents and materials

[0025] Glycine, disodium hydrogen phosphate, sodium dihydrogen phosphate, Tween-20 and protease inhibitors were all purchased from Sigma-Aldrich, USA. Bovine serum albumin (BSA) was purchased from Merck, Germany. Cy3 fluorescence was purchased from American Amerhsma Company. Other chemical reagents were of analytical grade and were not further purified before use. All experimental water was ultrapure water treated by Milli-Q50 pure water system (Millipore, USA). Sephadex G-25 column desalting was purchased from GE Healthcare, USA. Chip hybridization kit was purchased from Bio-Rad, USA. Other common glassware are all made in China. Thirty-seven lectins (see Table 1 for specific names) were purchased from Sigma-Aldrich a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an application of a lectin probe combination in identification of rat sex based on urine protein glycotype. The specific lectin probe combination is a combination with significant difference of AAL, DSA, Jacalin, EEL and PTL-II, and when a sample detection result shows that the expression of AAL, DSA and Jacalin is up-regulated and the expression of EEL and PTL-II is down-regulated, it shows that a corresponding main body is a male rat; otherwise, the rat is a female rat. The method for identifying the sex of the rat is quick, simple, convenient and accurate, and the implementation cost is low.

Description

technical field [0001] The invention relates to a method for identifying the sex of rats. Background technique [0002] In order to improve the productivity of domestic animals, sex control of mammals has become an important content in animal husbandry research. People often hope to produce animals of the expected sex, thereby promoting the advancement of animal genetics and breeding technology, strengthening the prevention and treatment of associated diseases, and better utilization of resources. thereby serving humanity itself. In the study of wild animals, gender is one of the basic contents of animal population ecology research, and the individual ratio of male and female animals plays an important role in revealing the population structure. [0003] With the development of molecular genetics, developmental biology and related disciplines, early sex identification methods include: X-correlation enzyme identification, chromosome karyotype analysis, H-Y antigen detection ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/533
CPCG01N33/68G01N33/543G01N33/533G01N2440/38
Inventor 郭松涛常鸿莉陈瑞白瑞峰祝丰何刚李保国
Owner NORTHWEST UNIV(CN)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com