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Device for recovering ammonium and concentrated protein stock solution from ammonia-containing protein stock solution and method thereof

A technology for concentrating protein and proprotein, applied in fertilization equipment, peptide preparation methods, chemical instruments and methods, etc., can solve the problems of complicated purification process, high cost, affecting the reuse of protein stock solution, etc., and improve the efficiency of deamination , low cost, and high accuracy of the amount added

Inactive Publication Date: 2020-10-09
GUANGDONG WENYANG ENVIRONMENTAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In the field of biotechnology, purification methods are generally used for the recovery of ammonium-containing protein stock solutions and the treatment of concentrated protein stock solutions. However, the existing purification methods are complicated and costly, and the presence of ammonia and nitrogen will affect the reuse of protein stock solutions.

Method used

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  • Device for recovering ammonium and concentrated protein stock solution from ammonia-containing protein stock solution and method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This embodiment provides a method for recovering ammonium from an ammonia-containing protein stock solution and concentrating the protein stock solution.

[0056] A method for recovering ammonium from an ammonia-containing protein stock solution and concentrating the protein stock solution, comprising the steps of:

[0057] The ammonia-containing protein stock solution enters the removal mechanism 200 after adjusting the pH value to a predetermined range, and the removal mechanism 200 corrects the NH in the ammonia-containing protein stock solution. 4 + Convert to NH 3 to achieve removal.

[0058] The protein stock solution from the removal mechanism 200 enters the MVR forced circulation concentration mechanism 400 for concentration to obtain high-concentration protein stock solution and distilled water;

[0059] Containing NH from the removal mechanism 200 3 The gas enters the ammonia absorption mechanism 300 for adsorption, and the NH in the gas 3 Converted to (N...

Embodiment 2

[0061] This embodiment provides a method for recovering ammonium from an ammonia-containing protein stock solution and concentrating the protein stock solution.

[0062] A method for recovering ammonium from an ammonia-containing protein stock solution and concentrating the protein stock solution, comprising the steps of:

[0063] see figure 1 As shown, the ammonia-containing protein stock solution is input into the adjustment mechanism 100, the initial protein content of the ammonia-containing protein stock solution is 1.0-1.2%, the pH value is 10-12, and the ammonia nitrogen content is 1200-2500 mg / L.

[0064] The ammonia-containing protein stock solution enters the removal mechanism 200 after adjusting the pH value to 11.5-12 in the adjustment mechanism 100, and the removal mechanism 200 corrects the NH in the ammonia-containing protein stock solution 4 + Convert to NH 3 to achieve removal.

[0065] The protein stock solution from the removal mechanism 200 enters the MV...

Embodiment 3

[0068] This embodiment provides a method for recovering ammonium from an ammonia-containing protein stock solution and concentrating the protein stock solution.

[0069] A method for recovering ammonium from an ammonia-containing protein stock solution and concentrating the protein stock solution, comprising the steps of:

[0070] see figure 1 As shown, the ammonia-containing protein stock solution is input into the regulating mechanism 100, the initial protein content of the ammonia-containing protein stock solution is 1.0%, the pH value is 10, and the ammonia nitrogen content is 1200 mg / L.

[0071]The ammonia-containing protein stock solution enters the removal mechanism 200 after adjusting the pH value to 12 in the adjustment mechanism 100, and blows air to the removal mechanism 200 through the blower, and the ammonia-containing protein stock solution realizes NH in the removal mechanism 200. 4 + Convert to NH 3 To achieve removal, and to obtain protein stock solution. ...

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Abstract

The invention discloses a device for recovering ammonium and a concentrated protein stock solution from an ammonia-containing protein stock solution and a method thereof. The device for recovering theammonium from the ammonia-containing protein stock solution and concentrated protein stock solution comprises an adjusting mechanism, a removing mechanism, an ammonia absorption mechanism and an MVRforced circulation concentration mechanism, wherein the adjusting mechanism, the removing mechanism and the MVR forced circulation concentration mechanism are connected in sequence; the ammonia absorption mechanism is connected with the removal mechanism; the adjusting mechanism is used for adjusting the pH value of the ammonia-containing protein stock solution; the removal mechanism is used for converting NH4<+> in the ammonia-containing protein stock solution into NH3 so as to realize removal and obtain a protein stock solution; wherein the ammonia absorption mechanism is used for adsorbingNH3 from the removal mechanism and converting the NH3 into ammonia sulfate, and the MVR forced circulation concentration mechanism is used for concentrating a protein stock solution from the removal mechanism to obtain a high-concentration protein stock solution and distilled water. The device for recovering the ammonium from the ammonia-containing protein stock solution and the concentrated the protein stock solution has the advantages of low cost, good ammonium recovery effect and good protein stock solution purification effect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a device and method for recovering ammonium from ammonia-containing protein stock solution and concentrating protein stock solution. Background technique [0002] In the field of biotechnology, purification methods are generally used for the recovery of ammonium and the treatment of concentrated protein stock solutions containing ammonia. However, the existing purification methods are complicated and costly, and the presence of ammonia and nitrogen will affect the reuse of protein stock solutions. Contents of the invention [0003] Based on this, it is necessary to provide a device and method for recovering ammonium from ammonia-containing protein stock solution and concentrating protein stock solution with good ammonium recovery effect and good protein stock solution purification effect. [0004] A device for recovering ammonium and concentrated protein stock solution containing a...

Claims

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Application Information

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IPC IPC(8): C07K1/14C01C1/242C05C3/00
CPCC01C1/242C05C3/00C07K1/14
Inventor 叶伟炳王明富
Owner GUANGDONG WENYANG ENVIRONMENTAL TECH CO LTD
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