Method for judging suitable technological parameters of low-temperature long-time cooking based on protein thermal denaturation degree
A technology of process parameters and thermal denaturation, applied in the preparation of test samples, material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of large sample consumption and cumbersome identification process. and other problems, to achieve the effect of less sample consumption and simple identification process
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[0053] Heating at 55°C for 4h, 8h, and 24h are three typical low-temperature long-time cooking process parameters, so three sets of cooking process parameters are designed, which are heating at 55°C for 4h, heating at 55°C for 8h, and heating at 55°C for 24h. The samples were steamed at low temperature for a long time to prepare three groups of meat samples to be identified, and the degree of protein thermal denaturation of each group of meat samples to be identified was measured X 1 、X 2 and x 3 , the specific measurement steps are as follows:
[0054] x 1 Determination: Step a, take 2g of meat sample and add 20mL of 20mM phosphate buffer (pH=6), and use a high-speed homogenizer to homogenize at 20600rpm for 30s; Adjust to 5 mg / mL; step c, take 1 mL of the diluted protein solution obtained in step b and add 200 μL of 1 mg / mL bromophenol blue BPB solution as the test group, and directly add BPB solution into the phosphate buffer as the control group; step d 1. Place the so...
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