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Molecular probe APT10-3.2-MZF-NPs for diagnosing prostatic cancer and preparation method of molecular probe APT10-3.2-MZF-NPs

A molecular probe, prostate cancer technology, applied in the field of medical detection, can solve the problems of lack of diagnostic specificity, short cycle time, lack of high sensitivity of prostate cancer, etc.

Inactive Publication Date: 2020-09-15
JIANGSU TAIZHOU PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing MRI technology is playing an increasingly important role in the diagnosis, grading and follow-up of prostate cancer, but it still lacks high sensitivity for the diagnosis of early prostate cancer, especially low-grade and small-sized prostate cancer
In addition, currently the most widely used clinically is the gadolinium-based T1 relaxation contrast agent (such as Gd-DTPA). Although this type of contrast agent can enhance the contrast between tissues, it lacks diagnostic specificity and circulates in the body. The time is short, thus limiting its application in tumor diagnosis

Method used

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  • Molecular probe APT10-3.2-MZF-NPs for diagnosing prostatic cancer and preparation method of molecular probe APT10-3.2-MZF-NPs
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  • Molecular probe APT10-3.2-MZF-NPs for diagnosing prostatic cancer and preparation method of molecular probe APT10-3.2-MZF-NPs

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Embodiment Construction

[0016] 1. Preparation, characterization and targeting study of APT10-3.2-MZF-NPs

[0017] 1. Preparation and characterization of APT10-3.2-MZF-NPs

[0018] 1) Preparation of PEG-MZF-NPs

[0019] The high-temperature pyrolysis method in the reference literature (Guo Ting. In vitro experimental research on the targeted diagnosis and treatment of ovarian cancer by shRNA / DDP magnetic nanoliposomes based on MFH technology [M]: [Master's Dissertation], Nantong: Nantong University, 2016) , to prepare PEG-MZF-NPs;

[0020] 2) Preparation of aptamer A10-3.2

[0021]According to literature (Wu M, Wang Y, Wang Y, et al.Paclitaxel-loaded and A10-3.2aptamer-targeted poly(lactide-co-glycolic acid) nanobubbles for ultrasound imaging and therapy of prostate cancer[J].Int J Nanomedicine, 2017,12:5313-5330), the nucleic acid sequence of A10-3.2 was prepared to prepare the aptamer A10-3.2.

[0022] 3) Preparation of APT10-3.2-MZF-NPs

[0023] Pipette 5 mL of the prepared PEGylated MZF-NPs (...

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Abstract

The invention belongs to the field of medical care detection, and particularly relates to a molecular probe APT10-3.2-MZF-NPs for diagnosing prostatic cancer and a preparation method of the molecularprobe APT10-3.2-MZF-NPs. The probe is prepared by coupling an aptamer A10-3.2 with manganese-zinc ferrite nanoparticles. The APT10-3.2-MZF-NPs molecular probe has specificity targeting on prostatic cancer LNCaP cells, can pass MR detection, and is hopeful to become a novel targetting contrast to be used for MR molecular imaging.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a molecular probe APT10-3.2-MZF-NPs for diagnosing prostate cancer and a preparation method thereof. Background technique [0002] Prostate cancer (PCa) is a malignant tumor of the urinary system with a very high incidence in European and American men. Although the incidence of prostate cancer in my country is lower than that of developed countries in Europe and America, due to changes in lifestyle and aging population in recent years, the incidence of prostate cancer has been increasing year by year, and there is a trend of younger age. The onset of prostate cancer is hidden, and the existing detection methods lack high sensitivity and specificity. Many patients have reached the advanced or advanced stage when they are initially treated, thus missing the best time for treatment. Androgen deprivation therapy (ADT) is the classic treatment for advanced prostate cancer...

Claims

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Application Information

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IPC IPC(8): A61K49/10
CPCA61K49/10
Inventor 林梅冯小倩
Owner JIANGSU TAIZHOU PEOPLES HOSPITAL
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