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Method for detecting alcohol metabolites of butadiene by using fluorescence derivatization-high performance liquid chromatography

A high-performance liquid chromatography and fluorescence derivatization technology, which is applied in the field of detecting alcohol metabolites of butadiene, can solve the problem of no suitable method for detecting compounds, etc., and achieves the effect of great application prospects.

Active Publication Date: 2020-09-11
SHANGHAI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problems of the prior art, the purpose of the present invention is to overcome the deficiencies in the prior art, provide a method for detecting the alcohol metabolites of butadiene using fluorescence derivatization-high performance liquid chromatography, and solve the problem that there is no suitable method for detection The problem with these compounds

Method used

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  • Method for detecting alcohol metabolites of butadiene by using fluorescence derivatization-high performance liquid chromatography
  • Method for detecting alcohol metabolites of butadiene by using fluorescence derivatization-high performance liquid chromatography
  • Method for detecting alcohol metabolites of butadiene by using fluorescence derivatization-high performance liquid chromatography

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Embodiment 1

[0062] In this embodiment, a method for detecting the alcohol metabolites of butadiene using fluorescence derivatization-high performance liquid chromatography, draws the working curves of CHB, E-CBol and Z-CBol and obtains the detection limit, comprising the following steps :

[0063] S1: Prepare the mixed solution of CHB, E-CBol and Z-CBol in PBS:

[0064] Dilute the DMSO stock solution of each compound with PBS to the range of 1-50 μM, set the concentration as 1, 5, 10, 25 and 50 μM and the blank control group, each 300 μL, set up three parallel samples;

[0065] Prepare CH with 10mg / mL FAA and 7.3mg / mL TEA 2 Cl 2 mixture:

[0066] Weigh 10mg FAA, dissolve in 1mL CH 2 Cl 2 , add 10 μL TEA; 3 mg / mL METS CH 2 Cl 2 Solution: 30mg METS dissolved in 10mL CH 2 Cl 2 solution; CH at 2.5 mg / mL DMAP 2 Cl 2 Solution: 25mg DMAP dissolved in 10mL CH 2 Cl 2 solution;

[0067] S2: Sample extraction:

[0068] Take 300 μL of the mixed solution of CHB, E-CBol and Z-CBol in PBS...

Embodiment 2

[0081] This embodiment is basically the same as Embodiment 1, especially in that:

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Abstract

The invention discloses a method for detecting alcohol metabolites of butadiene by using fluorescence derivatization-high performance liquid chromatography. The invention discloses a fluorescence derivatization-high performance liquid chromatography detection method for alcohol metabolites (including 1-chloro-3-butene-2-ol, trans and cis-4-chloro-2-butene-1-ol, and 3-butene-1, 2-diol) of 1, 3-butadiene (butadiene for short). The method belongs to the field of chemical detection methods, and comprises the following steps of carrying out liquid-liquid extraction on alcohol metabolites of butadiene in a sample, carrying out derivatization through 9-fluorenylacetic acid, sym-trimethylsulfonyl chloride and 4-dimethylaminopyridine, carrying out high performance liquid chromatography-fluorescencedetection, and quantifying by using an external standard method. Through verification, the fluorescence derivatization-high performance liquid chromatography method disclosed by the invention has thecharacteristics of strong specificity, high accuracy and the like, provides a reliable tool for researching alcohol metabolites of butadiene, and has a certain practical application value.

Description

technical field [0001] The invention relates to a method for detecting alcohol metabolites of butadiene, which is applied in the technical field of analytical chemistry. Background technique [0002] Butadiene is a colorless gas with a faint aromatic odor and is an important petrochemical raw material. Butadiene is produced by fossil fuel combustion and biomass combustion. Butadiene is found in a wide range of sources in the environment, such as automobile exhaust, forest fires and cigarette smoke. This also results in the ubiquity of butadiene in the environment, with concentrations ranging from 0.04-0.9 ppb. Butadiene is a carcinogen and has a high risk of cancer. Occupational exposure to butadiene has been associated with cancers of the lymphohematopoietic system. [0003] At present, the carcinogenic mechanism of butadiene is not clear, but it has been determined that butadiene itself is not carcinogenic, and its carcinogenicity comes from metabolites. Butadiene is m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74G01N21/64
CPCG01N30/06G01N30/74G01N21/6402
Inventor 李炯函相明辉张新宇栾洋
Owner SHANGHAI UNIV
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