Vertical microbiological detection chip

Abstract

The invention relates to a vertical microbiological detection chip. The vertical microbiological detection chip includes a conveying channel bent and extended between a chip inlet and a chip outlet, and an accommodating cavity formed in a horizontal part of the conveying channel, the bottom of the accommodating cavity is provided with a side flow channel of which the outlet is communicated with anext stage horizontally-arranged distribution channel, and a vertically-arranged chip allows an excitation light source and a fluorescence detection element to be arranged on two sides of the chip, sothat the background interference of excitation light can be completely shielded; the inlet end and the outlet end of the side flow channel are respectively communicated with the bottom of the accommodating cavity and a next stage distribution channel, so that micro-droplets can be induced under the condition of not consuming continuous phase fluid; the side flow channel can be independently arranged on a structural layer, so that the induction effect of the filled accommodating cavity on droplets is reduced or even eliminated; polarization coatings with opposite polarization directions are coated on the outer sides of a chip cover plate layer and a substrate layer, therefore, a light path between the excitation light and the fluorescence detection element is completely blocked, meanwhile,external polarization elements arranged on two sides of the chip are cooperated, a light path between a fluorescence signal emitted from the side wall of the accommodating cavity and the fluorescencedetection element can be blocked, and signal fusion between adjacent fluorescence signal points is avoided.

Description

Technical field [0001] The invention relates to a biological detection device, in particular to a vertical microbial detection chip that performs specific DNA detection of viruses, bacteria and other microorganisms through PCR reaction. Background technique [0002] The digital PCR chip based on microdroplets is one of the most advanced nucleic acid quantitative detection methods. Compared with traditional PCR technology, digital PCR will dilute the solution containing the target gene, primers, polymerase, etc. into dozens to dozens Tens of thousands of tiny, independent reactors, so that the number of nucleic acid templates in each reactor is less than or equal to one, and each reactor is subjected to traditional PCR amplification and fluorescence detection. Mark the reactor with the target gene as 1, and the reactor without the target gene as 0. According to the relative proportion and the volume of the reactor, and use the Poisson distribution to calculate the nucleic acid con...

AI Technical Summary

Problems solved by technology

However, due to the resistance of the microchannel itself and the local barrier, there is a large pressure difference in the continuous phase fluid flowing between the upstream and downstream of the distribution channel, which will lead to a huge difference in the filling effect of the micro-reaction chamber at the upstream and downstream of the distribution channel.

[0005] In addition, in the process of fluorescence detection of the amplified micro-reaction chamber, the reflection and refraction of the incident excitation light often cause strong background interference; Due to the non-directional nature of the fluorescence emitted by the stimulated micro-droplets in the reaction chamber, some of the fluorescence signals between adjacent micro-wells often pass through the hole wall and are mixed with the fluorescent signals from adjacent droplets, making it difficult to distinguish The problem

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