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Diff-Quik dye liquor special for animals and preparation method thereof

A dyeing solution and animal technology, which is applied in the field of medical biological detection, can solve the problems of insufficiently clear dyeing color and low differentiation of different cells, and achieve the effect of improving efficiency, clear dyeing color, and dyeing time advantage

Pending Publication Date: 2020-09-04
嘉兴优瑞生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The currently commercially available Diff-Quik staining solution is mainly used for the detection of human cells, although it can also be used for animal blood smears, but after it is applied to animal blood cell smears, the staining color is not clear enough and the staining background is visible Disadvantages of a small amount of sediment and poor differentiation of different cells

Method used

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  • Diff-Quik dye liquor special for animals and preparation method thereof
  • Diff-Quik dye liquor special for animals and preparation method thereof
  • Diff-Quik dye liquor special for animals and preparation method thereof

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preparation example Construction

[0040] The present invention also provides a kind of preparation method of special Diff-Quik dye solution for animals, comprising the following steps:

[0041] (1) Prepare a methanol solution containing Fast Green;

[0042] (2) Prepare the first aqueous solution containing eosin Y, potassium dihydrogen phosphate, and disodium hydrogen phosphate dodecahydrate;

[0043] (3) Prepare the second aqueous solution containing potassium dihydrogen phosphate and disodium hydrogen phosphate dodecahydrate, add azure II, azure I, eosin Y, and methylene blue to the second aqueous solution at 20~30°C, and dissolve completely Finally, adjust the pH of the mixed solution to 3.0~3.3, raise the temperature of the mixed solution to above 85°C, add potassium permanganate, and then react according to the following process:

[0044] Isothermal reaction at 90~95℃ for more than 50min;

[0045]Raise the temperature at 1°C / min to 98~100°C, keep warm for 5~10min;

[0046] Cool down to 60-70°C at 1°C / m...

Embodiment 1

[0063] Embodiment 1 of the present invention provides a special Diff-Quik dye solution for animals, including a first reagent, a second reagent and a third reagent;

[0064] The first reagent contains 1 mg of Fast Green and 1000 mL of methanol (HPLC);

[0065] The second reagent contains the following ingredients in parts by weight: 1.2g of eosin Y, 1.8g of potassium dihydrogen phosphate, 2g of disodium hydrogen phosphate dodecahydrate, 1000mL of ultrapure water, and 0.05g of preservative;

[0066] The third reagent contains the following ingredients in parts by weight: 1.2g of methylene blue, 1g of azure II, 1.2g of azure I, 0.03g of eosin Y, 1.8g of potassium dihydrogen phosphate, 2g of disodium hydrogen phosphate dodecahydrate, potassium permanganate 2g, ultrapure water 1000mL, preservative 0.05g.

[0067] The preparation process of the first reagent, the second reagent and the third reagent is as follows:

[0068] Accurately weigh 1 mg of fast green dye powder, pour it i...

Embodiment 2

[0072] Embodiment 2 of the present invention provides a special Diff-Quik dye solution for animals, including a first reagent, a second reagent and a third reagent;

[0073] The first reagent contains 2 mg of Fast Green and 1000 mL of methanol (HPLC);

[0074] The second reagent contains the following ingredients in parts by weight: 1.9 g of eosin Y, 2.8 g of potassium dihydrogen phosphate, 2.8 g of disodium hydrogen phosphate dodecahydrate, 1000 mL of ultrapure water, and 0.15 g of preservative;

[0075] The third reagent contains the following components by weight: 1.8g of methylene blue, 1.8g of azure II, 1.8g of azure I, 0.04g of eosin Y, 1.2g of potassium dihydrogen phosphate, 2.8g of disodium hydrogen phosphate dodecahydrate, permanganese Potassium acid 2.5g, ultrapure water 1000mL, preservative 0.15g.

[0076] The preparation process of first reagent, second reagent is with embodiment 1, and the 3rd reagent is prepared by following process:

[0077] Measure 900 mL of ...

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Abstract

The invention discloses a Diff-Quik dye liquor special for animals and a preparation method of the Diff-Quik dye liquor. The Diff-Quik dye liquor comprises a first reagent, a second reagent and a third reagent; the first reagent contains the following components: solid green and methanol; the second reagent contains the following components: eosin Y, monopotassium phosphate, disodium hydrogen phosphate dodecahydrate and ultrapure water; and the third reagent contains the following components: methylene blue, azure II, azure I, eosin Y, monopotassium phosphate, disodium hydrogen phosphate dodecahydrate, potassium permanganate and ultrapure water. The Diff-Quik dye liquor is suitable for animal blood cell smears, the staining color is very clear, the staining background is free of sediment,the color is bright, the distinction degree is high after staining of different cells, and the application range is wider; compared with the existing Diff-Quik dye liquor, the Diff-Quik dye liquor disclosed by the invention has more advantages in dyeing time, and dyeing can be generally completed within 30 to 60 seconds. And the Diff-Quik dye liquor disclosed by the invention is extremely short inpreparation time, so that the production benefit is remarkably improved.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, [0002] In particular, the present invention relates to a special Diff-Quik dye solution for animals and a preparation method thereof. Background technique [0003] Diff-Quik staining is a rapid staining method improved on the basis of Wright Stain, and it is one of the commonly used staining methods in cytological examination. The staining solution is prepared by the rapid staining method recommended by the World Health Organization (WHO). It is similar to Wright Stain and is improved by using the principle of Wright Stain technology. The staining result is also very similar to Wright staining solution, but Diff-Quik staining The time required is very short, and the dyeing can be completed within 90 seconds. Diff-Quik staining is mainly used for blood cell smear, bone marrow smear, vaginal secretion smear, exfoliated cell smear, etc. [0004] The currently commercially a...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N21/84
CPCG01N1/2813G01N21/84G01N2001/302
Inventor 胡卫江丁蓉龙李建卫孙菠
Owner 嘉兴优瑞生物科技有限公司
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