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Preparation method of complex microbial inoculant for aquatic products

A compound bacterial agent and compound bacteria technology, applied in chemical instruments and methods, animal husbandry wastewater treatment, special compound water treatment, etc. Antagonistic effect, increase the type of bacteria, the effect of obvious effect

Inactive Publication Date: 2020-09-01
JINAN WEISHENGUI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the production process of water quality environment improvers for aquatic products, it is necessary to use various strains such as lactic acid bacteria, yeast, photosynthetic bacteria, and acetic acid bacteria for compounding, but there must be mutual antagonism between the multiple strains, resulting in the amplification The fermentation system is too different from the initially added strain system, most of the strains will be inhibited, and only a few strains will reproduce, which will greatly reduce the efficiency of the water quality environment improver produced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The present embodiment provides a kind of preparation method of composite bacterial agent for aquatic products, comprising the following steps:

[0017] Step 1: Boil 1% corn flour, 1% soybean meal, and 1% rice, mix and add 1% yeast extract powder, 1% fructose syrup, 1% molasses, 0.1% sodium chloride, 0.1% dipotassium hydrogen phosphate, 0.1% kelp powder, 0.1% magnesium sulfate, and put this mixed system into a dialysis bag;

[0018] Step 2: Place the dialysis bag in a high-quality water culture environment, and let it stand for 1 day;

[0019] Step 3: After the static culture is completed, use 1% yeast extract powder, 1% fructose syrup, 1% molasses, 0.1% sodium chloride, 0.1% dipotassium hydrogen phosphate, 0.1% kelp powder, 0.1% magnesium sulfate was used to expand the strains, and the expansion process was divided into two times.

Embodiment 2

[0021] The present embodiment provides a kind of preparation method of composite bacterial agent for aquatic products, comprising the following steps:

[0022] Step 1: Boil 5% corn flour, 5% soybean meal, and 5% rice, mix and add 3% yeast extract powder, 5% fructose syrup, 5% molasses, 0.5% sodium chloride, 0.3% dipotassium hydrogen phosphate, 0.5% kelp powder, 0.3% magnesium sulfate, and put this mixed system into a dialysis bag;

[0023] Step 2: Place the dialysis bag in a high-quality water culture environment, and let it stand for 1 day;

[0024] Step 3: After the static culture is completed, use 3% yeast extract powder, 5% fructose syrup, 5% molasses, 0.5% sodium chloride, 0.3% dipotassium hydrogen phosphate, 0.5% kelp powder, 0.3% magnesium sulfate was used to expand the strains, and the expansion process was divided into two times.

[0025] Compared with Example 1, the main feature is that the amount of culture medium is increased.

Embodiment 3

[0027] The present embodiment provides a kind of preparation method of composite bacterial agent for aquatic products, comprising the following steps:

[0028] Step 1: Boil 1% corn flour, 1% soybean meal, and 1% rice, mix and add 1% yeast extract powder, 1% fructose syrup, 1% molasses, 0.1% sodium chloride, 0.1% dipotassium hydrogen phosphate, 0.1% kelp powder, 0.1% magnesium sulfate, and put this mixed system into a dialysis bag;

[0029] Step 2: Place the dialysis bag in a high-quality water culture environment and let it stand for 7 days;

[0030] Step 3: After the static culture is completed, use 1% yeast extract powder, 1% fructose syrup, 1% molasses, 0.1% sodium chloride, 0.1% dipotassium hydrogen phosphate, 0.1% kelp powder, 0.1% magnesium sulfate was used to expand the strains, and the expansion process was divided into two times.

[0031] Compared with the second embodiment, the main feature is: the length of static culture has been increased.

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PUM

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Abstract

The invention discloses a preparation method of a complex microbial inoculant for aquatic products. The preparation method comprises the following steps: preparing a complex bacterium collection medium, collecting complex bacteria, and carrying out expanding culture on the complex bacteria. The complex bacterium collection medium is prepared from, by mass, 1%-10% of corn flour, 1%-10% of soybean meal, 1%-10% of rice, 1%-5% of yeast extract powder, 1%-10% of high fructose corn syrup, 1%-10% of molasses, 0.1%-1% of sodium chloride, 0.1%-0.5% of dipotassium phosphate, 0.1%-1% of kelp powder and 0.1%-0.5% of magnesium sulfate. The collection environment of complex bacteria is a healthy aquaculture water area, and the thallus collection time is 1-30 days. Yeast extract powder accounting for 1%-5% by mass, high fructose corn syrup accounting for 1%-10% by mass, molasses accounting for 1%-10% by mass, sodium chloride accounting for 0.1%-1% by mass, dipotassium phosphate accounting for 0.1%-0.5% by mass, kelp powder accounting for 0.1%-1% by mass and magnesium sulfate accounting for 0.1%-0.5% by mass are used for expanding culture of the complex bacteria. Compared with a traditional single-strain compounding method, the method has the advantages that the technical process is simpler, the strain components are more accurate, meanwhile, the mutual antagonism among strains is effectivelyavoided, and the strain types in the fermentation liquor are increased.

Description

technical field [0001] The invention relates to the technical field of production of water quality improvers, in particular to a method for preparing a composite bacterial agent for aquatic products. Background technique [0002] In the aquaculture industry, due to the excessive addition of exogenous antibiotics, feces and other pollutants, the number of beneficial microorganisms in the water body has decreased sharply, and the number of harmful microorganisms has increased sharply, which has caused serious deterioration of the water body environment, resulting in a decline in aquaculture production and poor quality. And other consequences, which makes the aquaculture environment not circular, causing farmers to suffer economic losses. [0003] Based on the above facts, a water quality environment improver for aquatic products was born. A small amount of aquatic probiotics is artificially added, which can use the carbon and nitrogen sources of the poor water quality environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F103/20
CPCC02F3/34C02F2305/06C02F2103/20
Inventor 汤传忠潘信磊刘毅聂相涛
Owner JINAN WEISHENGUI BIOLOGICAL TECH CO LTD
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