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Application of guggulsterone in preparation of medicine for treating psoriasis

A technology of myrrh sterone and psoriasis, applied in the field of drug development, can solve the problems of unclear cell proliferation and growth, and achieve the effects of aggravating the symptoms of psoriasis, inhibiting proliferation and growth, and inhibiting proliferation.

Inactive Publication Date: 2020-08-25
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes how certain compounds are able to prevent cell division caused by specific molecules called histones. These chemical substances help regulate genetic activity during different stages of embryogenesis such as germination, stem elongations, mitotic spindles, wound healing processes, etc., which could lead to various types of dermatoid rashes like plaque buildup on affected areas. By controlling these reactions, this treatment may improve conditions associated with chronoaging diseases including Psoriasis vulgaris and other autoimmune disorders.

Problems solved by technology

This patented technical problem addressed in this patents involves identifying specific naturally occurring molecules called lignanins or pyrenols found within psoriama plaque during different stages of severely affecting pigments production and cellular dysfunction caused by innate responses like autoimmunization. Natural products derived from these sources may offer potential benefits over synthetic agents currently available treatings because they act differently than other chemical entities involved in conventional medical care systems.

Method used

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  • Application of guggulsterone in preparation of medicine for treating psoriasis
  • Application of guggulsterone in preparation of medicine for treating psoriasis
  • Application of guggulsterone in preparation of medicine for treating psoriasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Bisabolone has an inhibitory effect on the growth of keratinocytes cultured in vitro

[0054] Bisabolone was purchased from Selleck Chemicals, USA (product number S3792), and was dissolved in DMSO (dimethyl sulfoxide) to prepare a 0.1M (mol / L) mother solution and stored at -20°C. HaCaT cells (product number GDC106) were purchased from the China Center for Type Culture Preservation, and the cells were cultured using RPMI-1640 medium (Gibco, USA, product number 11875127) supplemented with 10% (V / V) fetal bovine serum (Gibco, USA). , Product No. 1099141), and add 100 U / mL of penicillin and 100 mg / mL of streptomycin. All cells were incubated at 37°C, CO 2 Conditioned culture with a partial pressure of 5% (volume percentage). MTT cell proliferation and cytotoxicity detection kit was purchased from Beyontian Biotechnology Co., Ltd. (product number C0009).

[0055] Thiazolium blue (MTT) experiment: HaCaT cells in the logarithmic growth phase were treated with 10×...

Embodiment 2

[0059] Example 2: Bisabolone inhibits clonal growth of keratinocytes cultured in vitro

[0060] Crystal violet staining solution was purchased from Biyuntian Biotechnology Co., Ltd. (product number C0121).

[0061] Colony formation experiment: The method of culturing HaCaT cells is the same as that in Example 1. Select HaCaT cells in the logarithmic growth phase, inoculate 5000 cells / well into a 6-well cell culture plate, set up 3 multiple wells, and place at 37°C, 5% CO 2 Cultivate in the incubator for about 24 hours, then add the RPMI-1640 complete medium with the final concentration of 2, 4 μM bisabolone respectively, set the untreated cell group, and then add 0.1% (volume fraction) drug solvent DMSO group to set As the blank control group, after 24 hours of treatment, at 37 ° C, 5% CO 2 Cultivate in the incubator for about 10-15 days. When most of the single cells in the blank control group form more than 50 monoclonal cells, discard the original medium, wash twice with ...

Embodiment 3

[0064] Example 3: Bisabolone inhibits keratinocyte DNA replication

[0065] Cell immunostaining fixative was purchased from Biyuntian Biotechnology Co., Ltd. (product number P0098), and EdU cell proliferation detection kit was purchased from Guangzhou Ruibo Biotechnology Co., Ltd. (product number C10310-1). The antifade agent was purchased from Invitrogen (Product No. P36931).

[0066] Detection of EdU cell proliferation: The method of culturing HaCaT cells is the same as that in Example 1. For the EdU method to detect DNA replication, refer to the kit instructions, select the cells in the logarithmic growth phase, and add the RPMI-1640 complete medium with the final concentration of 15, 30, and 50 μM bisabolone respectively, set up the untreated cell group, and then add 0.1% (volume fraction) drug solvent DMSO group was set as the blank control group, treated for 24 hours, added EdU and placed in 37 ° C, 5% CO 2 Incubate in the incubator for 20 minutes and wash once with PB...

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Abstract

The invention relates to an application of guggulsterone in preparation of a medicine for treating psoriasis. According to the invention, the guggulsterone is applied to the preparation of the psoriasis treatment medicine for the first time. Cytological experiment results prove that the guggulsterone can effectively inhibit proliferation of human immortalized keratinocyte HaCaT cells to induce apoptosis of the cells by inhibiting JAK-STAT and/or NF-kappa B pathways; at the same time, the guggulsterone can also significantly reduce the expression of psoriasis related genes CXCL1, CXCL5, IL-6, IL-8, TNF, S100A7, S100A8 and S100A9 in HaCaT cells. The application prospect of the guggulsterone in the psoriasis treatment is wide, and more drug choices are provided for treatment of psoriasis.

Description

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Claims

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Application Information

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Owner SHANDONG UNIV
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