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Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device

An anaerobic culture and soil technology, applied in biochemical cleaning equipment, biochemical equipment and methods, enzymology/microbiology equipment, etc., can solve the problems of destruction, complicated sampling process, heavy workload, etc., and achieve controllability Good, simple operation steps, convenient operation effect

Pending Publication Date: 2020-08-14
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] When people conduct research on anaerobic bacteria or soil anaerobic products, they need to perform anaerobic culture on the soil. Generally, the samples are place

Method used

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  • Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device
  • Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device
  • Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device

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Embodiment 1

[0042] figure 1 For the anaerobic culture device provided by the present invention, wherein, 1 is a sample bottle, 2 is a rubber stopper, 3 is a needle of a first syringe, 4 is a sample tube of a first syringe, 5 is a needle of a second syringe, and 6 is a sample of a second syringe Tube. according to figure 1 The connections shown result in an anaerobic culture setup.

[0043] use figure 1 Shown anaerobic cultivation device carries out soil anaerobic cultivation, comprises the following steps:

[0044] Described substratum adopts fresh water / seawater enrichment medium (FWM), with reference to the formula of Lovley (1986), in freshwater / seawater enrichment medium (FWM), add the amorphous iron oxide that final concentration is 60mmol / L, make simultaneously The pH of freshwater / seawater rich medium (FWM) was brought to 7.0 to obtain medium.

[0045] The composition of the fresh water / sea water enrichment medium is shown in Table 1.

[0046] Table 1 Composition of freshwate...

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Abstract

The invention provides an anaerobic culture device, a soil anaerobic culture method and a sampling method of the anaerobic culture device, and belongs to the technical field of anaerobic culture. Theanaerobic culture device provided by the invention is simple in structure, convenient to operate, economical, practical and convenient to carry to a field site for operation. Besides, the device is high in air tightness and simple in operation steps, ensures that a sample is cultured under an anaerobic condition, and is good in effect. Meanwhile, a first injector sample tube, a second injector sample tube and a sample bottle are provided with scales, so that the controllability is high. The soil anaerobic culture method provided by the invention is simple to operate, and the anaerobic environment is easy to create and maintain. The invention further provides the sampling method of the anaerobic culture device in the technical scheme, the sampling method avoids contact between the sample and oxygen in the sampling process, and the anaerobic environment is guaranteed.

Description

technical field [0001] The invention relates to the technical field of anaerobic cultivation, in particular to an anaerobic cultivation device, a soil anaerobic cultivation method, and a sampling method for an anaerobic cultivation device. Background technique [0002] When people conduct research on anaerobic bacteria or soil anaerobic products, they need to perform anaerobic culture on the soil. Generally, the samples are placed in independent serum bottles for culture. When sampling after anaerobic culture, they are all destructive sampling. The process is complicated and the workload is enormous. Therefore, it is hoped that a simple and airtight anaerobic culture device can be established to overcome the above problems. Contents of the invention [0003] The object of the present invention is to provide an anaerobic cultivation device, a soil anaerobic cultivation method, and a sampling method of the anaerobic device. The anaerobic cultivation device provided by the ...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12M1/24C12M1/00
CPCC12M23/08C12M23/38C12M33/04
Inventor 迟光宇燕国辉朱彬赵文斌黄斌
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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