Method for rapidly determining collection time window in gas chromatography-tandem mass spectrometry dynamic multi-reaction monitoring mode analysis method

Abstract

The invention relates to a method for rapidly determining a collection time window in a gas chromatography-tandem mass spectrometry dynamic multi-reaction monitoring mode analysis method by utilizingretention indexes, and belongs to the technical field of tobacco flavor component detection. The method is characterized in that the current retention time of a target compound is predicted accordingto the last actual measurement retention time of the target compound and the last and current actual measurement retention time of n-alkanes adjacent to the target compound so that a time window of each target compound in the dMRM method is accurately and quickly created. The method has the advantages of rapidness, convenience and accuracy, and the workload of retention time correction is greatlyreduced.

Description

technical field [0001] The invention belongs to the technical field of detection of tobacco aroma components, and in particular relates to a method for rapidly determining the acquisition time window in the analysis method of gas chromatography tandem mass spectrometry (GC-MS / MS) dynamic multiple reaction monitoring mode (dMRM). Background technique [0002] Tobacco aroma components include aldehydes, ketones, alcohols, phenols, acids, ethers, esters, lactones, alkenes, pyridines, pyrroles, pyrazines and other types of volatile and semi-volatile components. The content level and mutual ratio of these components have a key impact on the style and quality of tobacco leaves and cigarettes, and are important chemical components that affect the aroma, quantity and flavor of tobacco. With the advancement of analytical techniques, the influence of aroma components on the sensory quality of tobacco leaves has received more and more attention. However, the differences in the propert...

AI Technical Summary

Problems solved by technology

However, the differences in the properties of flavor components and the complexity of the tobacco matrix make the analysis and research of its key flavor components challenging.

At present, gas chromatography-mass spectrometry (GC-MS) combined with general-purpose commercial spectral libraries is mostly used to analyze tobacco aroma components. Due to the constraints of sample matrix interference and insufficient sensitivity of GC-MS, the number of compounds that can be accurately characterized is extremely limited. Previous studies covered less than one hundred flavor components of tobacco leaves

In previous studies, the retention index was mostly used in the GC-MS analysis method to assist in the qualitative analysis of the target components on the basis of database retrieval, but the use of the retention index to determine the acquisition time window of the multi-target GC-MS / MS method has not been reported yet.

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