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Proliferation culture medium formula for culture of thick and strong tetraploid paulownia seedlings and application

A proliferation medium and tetraploid technology, applied in the biological field, can solve the problems of lowering the survival rate of transplanting seedlings, weak growth, weak growth of adventitious buds, etc.

Active Publication Date: 2020-07-07
SHANDONG FOREST SCI RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the multiplication coefficient is too high, there are too many clustered buds induced, and most of the adventitious buds grow weaker. If the formula is not adjusted in time in the next culture cycle, after these weak regenerated shoots are transferred to the rooting medium, the growth will be reduced. Weaker, resulting in reduced survival rate of transplanted seedlings

Method used

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  • Proliferation culture medium formula for culture of thick and strong tetraploid paulownia seedlings and application
  • Proliferation culture medium formula for culture of thick and strong tetraploid paulownia seedlings and application
  • Proliferation culture medium formula for culture of thick and strong tetraploid paulownia seedlings and application

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Experimental program
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Effect test

Embodiment 1

[0024]Cut the aseptic seedlings into about 2cm long bud stem segments, and transfer them to the proliferation medium for cultivating stout tetraploid Paulownia seedlings MS+IAA 0.05mg / L+6-BA 0.5mg / L+KT 0.05mg / L +NAA 0.05mg / L+(NH4)2HPO4 50mg / L +agar 4.5g / L+sucrose 30g / L (pH 5.8), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. After 20 days, induce to form thick and strong adventitious buds, and the multiplication factor is 3-5 times (see figure 1 ).

Embodiment 2

[0026] Cut the aseptic seedlings into about 2cm long bud stem segments, and transfer them to the proliferation medium for cultivating strong tetraploid paulownia seedlings MS+IAA 0.5mg / L+6-BA 3.0mg / L+KT 1.0mg / L +NAA 1.0mg / L+(NH4)2HPO4 200mg / L+ agar 5.0g / L+ sucrose 30g / L (pH 5.8), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d . After 20 days, induce to form thick and strong adventitious buds, and the multiplication factor is 5-8 times (see figure 2 ).

Embodiment 3

[0028] Cut the sterile seedlings into about 2cm long bud stem segments, and transfer them to the proliferation medium for cultivating strong tetraploid Paulownia seedlings MS+IAA 3.0mg / L+6-BA 7.0mg / L+KT 3.0mg / L +NAA 3.0mg / L+(NH4)2HPO4 500mg / L+ agar 6.0g / L+ sucrose 40g / L (pH 6.0), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d . After 20 days, induce to form thick and strong adventitious buds, and the multiplication factor is 3-6 times (see image 3 ).

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Abstract

The invention discloses a proliferation culture medium formula for culture of thick and strong tetraploid paulownia seedlings and an application. The formula is formed in a manner that an MS culture medium is added with 0.05-3.0 mg / L of indoleacetic acid, 0.5-7.0 mg / L of 6-benzylaminoadenine, 0.05-3.0 mg / L of kinetin, 0.05-3.0 mg / L of naphthylacetic acid, 50-500 mg / L of diammonium phosphate, 4.0-6.0 g / L of agar and 30-40 g / L of sucrose. The formula can remarkably promote adventitious buds regenerated by tetraploid paulownia to be thick, strong and high-quality, the amplification coefficient is3-9, rooting is easier, the survival rate of transplanted and hardened seedlings is improved, technical support is provided for industrialized production of excellent tetraploid paulownia varieties,and the problem of serious short supply of seedlings of the excellent tetraploid paulownia varieties is solved.

Description

technical field [0001] The invention relates to a formula and application of a proliferation medium for cultivating robust tetraploid paulownia seedlings, which is especially suitable for subgeneration and multiplication in the later stage of industrialized seedling cultivation, and belongs to the field of biotechnology. Background technique [0002] Paulownia is a plant of the genus Paulownia in the family Scrophulariaceae. It is native to my country. It has no flying catkins, beautiful trees, and a long history of cultivation. It is naturally distributed in 25 provinces, municipalities, and autonomous regions in my country. It is an important fast-growing timber and native tree species for landscaping in my country. The leaves, flowers, fruits and bark of Paulownia can be used as medicine, and the leaves and flowers of Paulownia can be used as feed. Paulownia wood is high-quality and high-grade, corrosion-resistant, acid and alkali resistant, wear-resistant, easy to proces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 毛秀红闫少波王迎毛欣刘泉华辉亓玉昆仲伟国李猛张新慰张倩王晓英
Owner SHANDONG FOREST SCI RES INST
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