Preparation method of high fibrin vegetarian meat
A protein liquid and protein technology, which is applied in the field of preparation of high-fibrin meat embryos, achieves the effects of inhibiting blood sugar rise, easy digestion and absorption, and strong functional effects
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Embodiment 1
[0049] The invention provides a method for preparing high-fibre protein meat, which specifically includes the following steps:
[0050] (1) The defatted hemp seed residue after hemp seed oil extraction is dried under 50°C hot air condition, crushed into 60-mesh granules, add 3 times of water and 0.05% vitamin C and 0.08% vitamin E (to prevent the oxidation of fat and phenolic substances), adjust the pH value to 9 with edible alkali, after stirring evenly, after soaking for 2h, centrifuge to collect the supernatant, and make the hemp seed protein extract; the protein extraction rate is 60%, The content is 9%;
[0051] (2) Pipette the third-generation lactic acid bacteria (Lactobacillus plantarum and Pediococcus) activated by the method of liquid MRS medium, wash them twice with PBS buffer (pH 7.2), and adjust the concentration of lactic acid bacteria to 1-2×10 8 cfu / mL. After mixing Lactobacillus plantarum and Pediococcus according to a volume ratio of 5:1, a fermented seed l...
Embodiment 2
[0056] The invention provides a method for preparing high-fibre protein meat, which specifically includes the following steps:
[0057] (1) The defatted hemp seed residue after the hemp seed is oil-pressed, after being dried under the hot air condition of 55 ℃, be pulverized into 40 mesh particles, add 4 times of water and 0.05% vitamin C and 0.10% vitamin E (to prevent the oxidation of fat and phenolic substances), adjust the pH value to 10 with edible alkali, after stirring evenly, after soaking for 3h, centrifuge and collect the supernatant to obtain the hemp seed protein extract; the protein extraction rate is 63%, The content is 9.5%;
[0058] (2) Pipette the third-generation lactic acid bacteria (Lactobacillus plantarum and Pediococcus) activated by the method of liquid MRS medium, wash them twice with PBS buffer (pH 7.2), and adjust the concentration of lactic acid bacteria to 1-2×10 8 cfu / mL. After mixing Lactobacillus plantarum and Pediococcus according to a volume ...
Embodiment 3
[0062] The invention provides a method for preparing high-fibre protein meat, which specifically includes the following steps:
[0063] (1) The defatted hemp seed residue after hemp seed oil extraction is dried under 45°C hot air condition, crushed into 50-mesh granules, and added 2.5 times of water and 0.05% vitamin C and 0.12% vitamin E (to prevent the oxidation of fat and phenolic substances), adjust the pH value to 10 with edible alkali, after stirring evenly, after soaking for 2.5h, centrifuge to collect the supernatant, and make the hemp seed protein extract; the protein extraction rate is 61% , with a content of 9.3%;
[0064] (2) Pipette the third-generation lactic acid bacteria (Lactobacillus plantarum and Pediococcus) activated by the method of liquid MRS medium, wash them twice with PBS buffer (pH 7.2), and adjust the concentration of lactic acid bacteria to 1-2×10 8 cfu / mL. After mixing Lactobacillus plantarum and Pediococcus according to the volume ratio of 4:1,...
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