Method and application for inducing generation of Brassica napus seed

Abstract

The invention relates to a method for inducing generation of Brassica napus seed. Brassica napus seed is used as a female parent and orychophragmus violaceus is used as a male parent, the stamens of the female parent plant were removed manually at the bud stage, and pollen from the male parent plant was used for pollination for female parent plant. After pollination, the inflorescence of the female parent plant is bagged until the final flower and the carob of the female parent plant are enlarged. Carobs are harvested during maturity to obtain seeds of parthenogenetic offspring of Brassica napus. The invention successfully induces the production of parthenogenetic seeds through distant hybridization of Brassica napus and orychophragmus. The plant parthenogenesis can spontaneously produce seeds having diploid embryos with maternal genetic composition, normal endosperm and seed coat in plants. The production of artificial seeds can be realized simply and efficiently; The invention realizes the production of asexual propagation seeds of Brassica napus. This technology can be carried out in the field, and the operation is simple.

Description

technical field [0001] The invention belongs to the technical field of propagation of Brassica napus strains, and in particular relates to the application of a method for inducing the production of Brassica napus seeds. Background technique [0002] Brassica napus is an oil-bearing crop of Brassica genus Brassicaceae. It is allotetraploid. Its flowers are hermaphroditic. The conventional reproduction method is to produce seeds through outcross pollination, that is, sexual reproduction. In order to make the genotype of the offspring consistent with the maternal parent, tissue culture techniques such as callus culture, anther culture and free microspore culture are usually adopted. For tissue culture, a tissue culture laboratory must be established, and a certain amount of experimental funds must be invested. 1. Cultivate personnel who master the experimental skills, the cost is high, and the yield of callus culture and anther culture is low; although the yield of microspore c...

AI Technical Summary

Problems solved by technology

Naturally occurring parthenogenesis also exists in Brassica napus, but the incidence rate is extremely low (0-0.364%), it is difficult to control, and it cannot be practically used in rapeseed production

For a long time, rapeseed breeders have been carrying out the common sense of parthenogenesis in rapeseed. For example, Luo Peng published a paper in 1981 and pointed out that it can be induced by cabbage type rape, Gao Guangdong published a paper in 1995 and said that the use of broad bean distant hybridization to induce cabbage type rapeseed parthenogenesis, but the results showed that their attempts not only obtained few distant hybrid seeds, but also obtained haploid and diploid co-existence of parthenogenetic progeny, making it difficult to obtain parthenogenetic progeny in large quantities stably. There is still a gap in the requirements of practical application

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