Isolation and culture method of mouse renal clear cell carcinoma circulating tumor cell line and human-derived clear cell renal carcinoma circulating tumor cell
A technology for renal clear cell carcinoma and tumor cells, which is applied in the directions of cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve the problems such as the separation and culture method of circulating tumor cells in renal cancer that have not yet been seen, and achieves a simple culture method. Easy to operate, strong proliferation, high purity effect
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[0031] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0032] The present invention is a mouse renal clear cell carcinoma circulating tumor cell line of an embodiment, which is a circulating tumor cell of orthotopic renal carcinoma NOD / SCID mouse
[0033] The method for separating and culturing circulating tumor cells of renal clear cell carcinoma according to the embodiment of the present invention is as follows:
[0034] (1) Reagents used in the experiment
[0035] Lentivirus EF1A-MCS-P2A-EGFR-SV40-firefly_Luc-IRES-Puromycin (Gibco Gene); Polybrene dye aid (Gibco Gene); red blood cell lysate (Biyuntian); epithelial growth factor EGF (Gibco); alkali ...
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