Separation and culture method of mouse kidney clear cell carcinoma circulating tumor cell line and human kidney clear cell carcinoma circulating tumor cells
A technology for renal clear cell carcinoma and tumor cells, applied in the direction of cell culture active agents, biochemical equipment and methods, tissue culture, etc., can solve problems such as separation and culture methods for circulating tumor cells of renal cancer that have not been seen yet, and achieve simple culture methods Easy to operate, strong proliferation, high purity effect
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[0031] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0032] The present invention is a mouse renal clear cell carcinoma circulating tumor cell line of an embodiment, which is a circulating tumor cell of orthotopic renal carcinoma NOD / SCID mouse
[0033] The method for separating and culturing circulating tumor cells of renal clear cell carcinoma according to the embodiment of the present invention is as follows:
[0034] (1) Reagents used in the experiment
[0035] Lentivirus EF1A-MCS-P2A-EGFR-SV40-firefly_Luc-IRES-Puromycin (Gibco Gene); Polybrene dye aid (Gibco Gene); red blood cell lysate (Biyuntian); epithelial growth factor EGF (Gibco); alkali ...
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