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Manufacturing method for artificial seeds of Delonix regia

A technology of artificial seeds and production methods, applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of increased investment, low germination rate, waste of seeds, etc., achieve low cost and increase germination rate and rooting rate, the effect of easy source

Inactive Publication Date: 2020-06-23
西安艾默农林生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a method for making artificial seeds with simple process and low cost,

Method used

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  • Manufacturing method for artificial seeds of Delonix regia
  • Manufacturing method for artificial seeds of Delonix regia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The making method of poinciana artificial seed comprises the following steps:

[0035] Step 1, the collection of seeds, collect the seeds of the year, and select the seeds with the same size, full and disease-free;

[0036] Step 2. UV sterilize the laboratory for 30 minutes before the experiment, use a pressure cooker to sterilize the culture bottle, tweezers, tap water and pipette tips; prepare MS medium and the required hormone mother solution: 6-BA 10㎎ / L, NAA 10㎎ / L. The prepared medium needs to be autoclaved before use. The prepared hormone needs to be filtered through a water-soluble syringe filter and then placed in the refrigerator for freezing. Among them, the use conditions of the pressure cooker are 121°C and 18min;

[0037] Step 3. Soak the seeds collected in step 1 in tap water at normal temperature for 2 hours in advance to obtain seeds with swollen seed coats, and then carry out conventional disinfection treatment. That is, put the seeds under tap wate...

Embodiment 2

[0051] Step 1. Seed collection: Collect the seeds of the current year, and select the seeds that are consistent in size, plump and disease-free.

[0052] Step 2. Disinfection and preparation of the laboratory and experimental equipment before the experiment. The laboratory is sterilized by ultraviolet light for 30 minutes, and the culture bottle, tweezers, tap water and pipette tip are sterilized with a pressure cooker. The MS medium and the hormone mother solution were prepared, and the prepared MS medium was sterilized in a pressure cooker before use, and the use conditions of the pressure cooker were 121°C and 18 minutes. The prepared hormone is filtered through a water-soluble syringe filter and then placed in a refrigerator for freezing.

[0053] Step 3, seed treatment: soak the seeds collected in step 1 in tap water at normal temperature for 2 hours in advance to obtain seeds with swollen seed coats, and then carry out routine disinfection treatment, first rinse with tap...

Embodiment 3

[0059] Step 1. Seed collection: Collect the seeds of the current year, and select the seeds that are consistent in size, plump and disease-free.

[0060] Step 2. Disinfection and preparation of the laboratory and experimental equipment before the experiment. The laboratory is sterilized by ultraviolet light for 30 minutes, and the culture bottle, tweezers, tap water and pipette tip are sterilized with a pressure cooker. The MS medium and the hormone mother solution were prepared, and the prepared MS medium was sterilized in a pressure cooker before use, and the use conditions of the pressure cooker were 121°C and 18 minutes. The prepared hormone is filtered through a water-soluble syringe filter and then placed in a refrigerator for freezing.

[0061] Step 3, seed treatment: soak the seeds collected in step 1 in tap water at normal temperature for 2 hours in advance to obtain seeds with swollen seed coats, and then carry out routine disinfection treatment, first rinse with tap...

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Abstract

The invention discloses a manufacturing method for artificial seeds of Delonix regia. The specific steps of the manufacturing method are as follows: collecting seeds of the year, and performing disinfection treatment on the seeds; inoculating the disinfected seeds in a culture medium to cultivate so as to obtain Delonix regia seedlings; cutting 2-4 mm long stem sections with axillary buds as artificial seed embryos, and preparing artificial seed coats and artificial endosperm; adding 0.3% of carbendazim powder into 0.2-0.3 mol/L of a calcium chloride solution, and then adding 0.1 mg/L of penicillin so as to obtain an A solution; adding 3-5 mg/L of 6-BA and 8-12 mg/L of NAA into 2-4% of a sodium alginate solution so as to obtain a B solution; and adopting a hydrogel method to embed the artificial seed embryos so as to obtain artificial seeds. The produced artificial seeds are small in volume, convenient in transportation and conservation and low in cost; the germination rate and rootingrate of Delonix regia seeds can be greatly enhanced; and the manufacturing method has good economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for making artificial seeds of poinsettia. Background technique [0002] Phoenix wood (scientific name: Delonix regia), named after "the leaves are like the feathers of a flying phoenix, and the flowers are like the crown of a red phoenix". ), fire tree, shadow tree, foreign couplet and fire tree etc. Poinciana is native to Madagascar, Africa, and is a world-famous landscaping tree species, which is commonly introduced and cultivated in tropical regions. Poinciana is a plant of the Fabaceae Poinciana genus. Deciduous tree, up to 20 meters high. The canopy is broad. Bipinnate compound leaves, leaflets oblong. Blooms in summer in racemes, large, red and shiny. Pods woody, up to 50 cm long. Poinciana is known as one of the most colorful trees in the world due to its bright red or orange flowers and bright green pinnate leaves. It stands out with its gor...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/006A01H4/001
Inventor 王小帆史玮
Owner 西安艾默农林生物技术有限公司
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