Fungus fluorescent staining solution and preparation method thereof

Abstract

The invention belongs to the technical field of biological examination of pathogenic bacteria in medical clinical examination, and particularly relates to a fungus fluorescent staining solution and apreparation method thereof. The fungus fluorescent staining solution is prepared from the following components in parts by weight: 0.5 to 1.5 parts of a fluorescent whitening agent, 0.07 to 0.15 partof a background pressure preparation, 10 to 15 parts of a dissolution promoter, 1 to 2.5 parts of an anti-fluorescence decay agent, 0.3 to 0.8 part of a surfactant and 0.5 to 1.5 parts of sodium chloride. The fluorescent whitening agent and the background dye are mixed and prepared; according to the staining solution, the anti-fluorescence decay agent, the surfactant, the dissolution promoter andthe sodium chloride are added, so that the staining effect and the staining durability of the staining solution are remarkably improved, the staining solution can be stably placed for a long time, specimen staining steps are simplified, and the staining solution has the advantages of high staining speed and good effects.

Description

technical field [0001] The invention belongs to the technical field of biological inspection of pathogenic bacteria in medical clinical inspection, and in particular relates to a fungal fluorescent staining solution and a preparation method thereof. Background technique [0002] Microscopic examination is a commonly used clinical method for detecting fungi, which has the advantages of simple steps and fast speed. As a commonly used microscopic examination method, the KOH method utilizes the principle that high concentration of alkali dissolves tissue cells but cannot dissolve the polysaccharides on the fungal cell wall, and distinguishes fungi by observing the morphology of fungi. Although the KOH fungal detection method is quick and easy, because KOH cannot completely dissolve the tissue, the contrast between fungi and tissue cells is not great, and it is very difficult to identify fungi. Only experienced and professionally trained inspectors can distinguish fungi from tiss...

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Problems solved by technology

However, the fungal fluorescent dyes in the prior art have the following disadvantages: (1) the fluorescent whitening agent has poor stability in a strong alkaline environment, and the fluorescein will precipitate and precipitate; (2) the background pressure agent will affect the stability of the fluorescein Sexuality, it will cause a decrease in detection sensitivity, and even the fungus cannot be stained. Therefore, the components of the staining solution cannot coexist stably for a long time, and they need to be prepared and stored separately. It takes two or even three steps to complete the staining. The operation is relatively cumbersome and the staining time is relatively long. Long; (3) The fluorescence effect is quickly quenched after staining, and the sample cannot be preserved

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