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Nano-enzyme as well as preparation method and application thereof

A nano-enzyme and gold nano-particle technology, applied in the field of enzyme catalysis, can solve the problems of instability, catalytic performance sensitivity, low natural enzyme content, etc., and achieve the effect of low energy consumption and significant antibacterial effect

Pending Publication Date: 2020-06-02
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most natural enzymes are proteins, which are prone to structural changes and lose their activity under non-physiological conditions such as acid, alkali, heat, etc., which makes their catalytic performance less sensitive to external factors, unstable, etc. , in addition, due to the low content of natural enzymes in the organism, the output is small, and the cost of synthesis and purification is high
Due to the above disadvantages, the wide application of natural enzymes is limited

Method used

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  • Nano-enzyme as well as preparation method and application thereof
  • Nano-enzyme as well as preparation method and application thereof
  • Nano-enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This embodiment provides a nanozyme, which comprises a bovine serum albumin skeleton and gold nanoparticles attached to the bovine serum albumin skeleton. Its preparation method is:

[0059] 200 mg of bovine serum albumin was dissolved in 15 mL of pure water, and then 10 mL of 25 mM chloroauric acid solution was mixed with it, stirred and reacted at 25° C. for 4 days, and then dialyzed for 24 hours with a 14,000 molecular weight dialysis bag to obtain the nanozyme.

Embodiment 2

[0061] This embodiment provides a nanozyme, which comprises a bovine serum albumin skeleton and gold nanoparticles attached to the bovine serum albumin skeleton. Its preparation method is:

[0062] 250 mg of bovine serum albumin was dissolved in 15 mL of pure water, and then 10 mL of 20 mM chloroauric acid solution was mixed with it, stirred and reacted at 20° C. for 7 days, and then dialyzed for 48 hours with a 12,000 molecular weight dialysis bag to obtain the nanozyme.

Embodiment 3

[0064] This embodiment provides a nanozyme, which comprises a bovine serum albumin skeleton and gold nanoparticles attached to the bovine serum albumin skeleton. Its preparation method is:

[0065] Dissolve 300 mg of bovine serum albumin in 15 mL of pure water, then mix 10 mL of 30 mM chloroauric acid solution with it, stir and react at 35° C. for 2 days, and then dialyze with a 14,000 molecular weight dialysis bag for 36 hours to obtain the nanozyme.

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Abstract

The invention relates to a nano-enzyme as well as a preparation method and application thereof. The nano-enzyme comprises a bovine serum albumin skeleton and gold nanoparticles attached to the bovineserum albumin skeleton. The nano-enzyme involved in the invention is a multifunctional nano-enzyme, has both oxidase activity and peroxidase activity, has potential application value in the fields ofreducing substance detection, enzyme-linked immunosorbent assay and the like, and has a remarkable antibacterial effect due to the fact that various free radicals can be produced.

Description

technical field [0001] The invention belongs to the technical field of enzyme catalysis, and in particular relates to a nanozyme and its preparation method and application. Background technique [0002] All life processes in nature are inseparable from enzymes. Natural enzymes are biomolecules with efficient catalytic functions that exist in almost all organisms. They play an important role in various biological processes in organisms and have high catalytic efficiency and high bottom. Due to its importance to biological processes and biological metabolism, it is often used in various biological practical applications, such as food industry, pharmaceutical technology and biomedicine, and has shown good application prospects. However, most natural enzymes are proteins, which are prone to structural changes and lose their activity under non-physiological conditions such as acid, alkali, heat, etc., which makes their catalytic performance less sensitive to external factors, uns...

Claims

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Application Information

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IPC IPC(8): B01J31/06C07K16/44G01N33/53A01N59/16A01N59/00A01P1/00G01N31/10
CPCB01J31/06C07K16/44G01N33/574A01N59/16A01N59/00G01N31/10B01J2231/70B01J35/40
Inventor 陈雯雯姚家健王纪东
Owner SHENZHEN UNIV
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