A kind of rna extraction device and method

Abstract

The invention belongs to the field of biotechnology, and specifically relates to an RNA extraction device and method, comprising a piston syringe, a plastic centrifuge tube, a centrifugal column arranged in the plastic centrifuge tube, a mixing centrifuge tube, the mixing centrifuge tube includes a tube body and a tube cover, A through hole is arranged on the tube cover, a silica gel column is arranged in the through hole, a top separation layer is arranged on the upper part of the inner cavity of the tube, and a middle separation layer is arranged on the middle and lower part, and the top separation layer and the middle separation layer separate the inside of the tube body. The chamber is divided into an upper chamber, a middle chamber and a lower chamber. Grinding balls are pre-placed in the upper chamber, TRIzol is pre-placed in the middle chamber, and chloroform is pre-placed in the lower chamber. In the process of extracting RNA in the present invention, the operator does not need to directly absorb and contact the organic solvent, and no toxic volatiles are released into the atmosphere during the operation, which protects the health of the experimenters; at the same time, the present invention realizes the detection of bulk tissue samples through grinding balls. Mechanical cutting and grinding of difficult-to-lyse tissue samples to release RNA more quickly and fully.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an RNA extraction device and method. Background technique [0002] RNA, also known as ribonucleic acid Ribonucleic Acid, is one of the key substances in the life process. Extracting highly pure and intact RNA helps researchers to fully understand the regulatory mechanisms in the process of RNA transcription, splicing and degradation. At present, RNA extraction mainly includes silica gel binding method, magnetic bead binding method, CTAB or PVP method, cesium chloride density gradient centrifugation method, etc. Among them, the TRIzol method is one of the most widely used RNA extraction methods at present. This method has significant advantages such as large RNA yield, low cost, and good versatility. It is widely used in different tissues and tissues from animals, plants, fungi, bacteria, and viruses. Extract RNA from cells. [0003] TRIzol and chloroform are key reagen...

AI Technical Summary

Problems solved by technology

Treat proteinase K at 100 degrees Celsius for 10 minutes, and DNaseI at 65 degrees Celsius for 10 minutes can be completely inactivated, but long-term high-temperature heating will also lead to degradation and destruction of RNA samples; phenol-chloroform extraction is the most stringent removal and inactivation The method of proteinase K and DNase I, but this method is cumbersome and time-consuming; although the EDTA method can inactivate DNase I and reduce the activity of proteinase K by chelating metal ions, EDTA may chelate metal ions and affect subsequent reverse transcription and PCR reactions

Although RNA purification methods such as silica gel binding and magnetic bead binding can use silica gel columns and magnetic beads to bind RNA within a certain length, and then remove most of proteinase K and DNase I through the eluent, the surface of the silica gel column and the magnetic A small amount of proteinase K and DNaseI will remain on the surface of the beads, and the binding efficiency of conventional silica gel columns and magnetic beads to ultra-long and ultra-short RNA sequences is low, which interferes with the results of subsequent precision experiments

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