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Grape VyNRT1 gene as well as encoded protein and application thereof in breeding of drought-resistant varieties

A technology that encodes proteins and grapes, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of enhanced drought resistance

Active Publication Date: 2020-05-26
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the model plant Arabidopsis thaliana, more than 50 NRT1 family members have been discovered, but only 10 of them have been studied clearly. It can be seen that the research on the function of NRT1 family genes in grapes needs further exploration

Method used

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  • Grape VyNRT1 gene as well as encoded protein and application thereof in breeding of drought-resistant varieties
  • Grape VyNRT1 gene as well as encoded protein and application thereof in breeding of drought-resistant varieties
  • Grape VyNRT1 gene as well as encoded protein and application thereof in breeding of drought-resistant varieties

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1 Grape VyNRT1 gene expression analysis

[0017] After subculture of Yanshan grape tissue culture seedlings for 16 days, the seedlings with consistent growth and performance were selected for various stress treatments.

[0018] Drought treatment: Pull out the grape seedlings from the culture medium, place them on filter paper and expose them to a room temperature of (32±1)°C, a relative humidity of 55%, and a photoperiod of light 14h / darkness 10h. 2, 6, 12, 24h sampling.

[0019] Low temperature treatment: culture the tissue cultured seedlings at a temperature of (4±1)°C, a relative humidity of 75%, and a photoperiod of 14 hours of light / 10 hours of darkness, and take samples at 0, 2, 6, 12, and 24 hours.

[0020] Salt stress: add 20mL 100mmol·L -1 The NaCl solution was cultivated at a temperature of (25±1)°C, a relative humidity of 75%, and a photoperiod of 14 h light / 10 h dark, and samples were taken at 0, 2, 6, 12, and 24 h.

[0021] An equal volume of dis...

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Abstract

The invention relates to a grape VyNRT1 gene as well as an encoded protein and an application of the grape VyNRT1 gene in drought-resistant variety breeding, which belong to the technical field of plant genetic engineering. According to the invention, by utilizing a transgenic technology of a strong promoter (cauliflower mosaic virus 35S promoter) driving principle, an overexpression vector of a Yanshan grape VyNRT1 gene is transferred into arabidopsis thaliana, so that a transgenic arabidopsis thaliana plant is obtained; the experiments prove that compared with an arabidopsis thaliana plant with a transformed empty vector, accumulation of stress resistance related substances in transgenic arabidopsis thaliana and expression of drought resistance related genes are caused by overexpressionof the VyNRT1 gene, and the drought resistance of the transgenic plant is enhanced. Therefore, the grape VyNRT1 gene and the recombinant expression vector thereof can be used for breeding drought-resistant varieties of plants.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the grape VyNRT1 gene and its encoded protein and the application of the gene in drought-resistant variety breeding. Background technique [0002] Grapes are native to western Asia and are cultivated all over the world. About 95% of grapes around the world are concentrated in the northern hemisphere. The main producing areas in China are Xiaoxian County in Anhui, Turpan and Hotan in Xinjiang, Yantai in Shandong, Zhangjiakou, Xuanhua, Hebei Changli, Dalian, Xiongyue, Shenyang in Liaoning and Lumiao Township, Minquan, Yifeng and other places in Henan. During the growth and development of grapes, water has an important impact on the yield and quality of grapes. Water shortage will easily threaten the grape industry. Under the background of water scarcity, excavating drought-resistant grape resources and studying grape drought-resistant genes are of great scientifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/20A01H6/88
CPCC07K14/415C12N15/8273
Inventor 余义和张贺程杨芯贤李敏王磊磊郭大龙杨英军张国海
Owner HENAN UNIV OF SCI & TECH
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