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Separation preparation process and application of natural free radical scavenger in Saxifraga atrata Engl

A preparation process and free radical technology, applied in the fields of application, preparation of sugar derivatives, food ingredients containing natural extracts, etc., can solve the problems of natural free radical scavengers that have not been reported in the literature and have not been systematically studied. It is suitable for large-scale production, easy to prepare materials in batches, and low cost.

Active Publication Date: 2020-05-19
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for lower costs while maintaining good quality products by separating different components from each other through various techniques such as reversal phase precipitation, microparticle size columns, gel filtration, etc., without requiring expensive equipment like traditional methods. It achieves this by efficiently collecting these extracts during use and reuse them later on when needed again.

Problems solved by technology

The technical problem addressed by this patented technology is how to efficiently separate and prepare naturally occurring substances called flavonoids or other chemicals found within safflower plant extracts like astaxanthin which can help improve their health benefits when taken into humans through various ways such as smoking or drinking them over time without causing negative side effects.

Method used

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  • Separation preparation process and application of natural free radical scavenger in Saxifraga atrata Engl
  • Separation preparation process and application of natural free radical scavenger in Saxifraga atrata Engl
  • Separation preparation process and application of natural free radical scavenger in Saxifraga atrata Engl

Examples

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Effect test

Embodiment 1

[0048] The separation and preparation process of natural free radical scavenger in black saxifrage, the method specifically includes the following steps:

[0049] Step 1, extraction: take 120g of the whole herb of Saxifrage saxifrage and dry it in the shade, coarsely crush it and extract it according to the ratio of solid to liquid: 1g: 5mL of methanol, extract 4 times at room temperature, each time for 2 hours, filter and combine the filtrates, that is, filtrate A, The filtrate A is mixed according to the amount of polyamide: the amount of black saxifrage medicinal material = 1:0.5 and then dried under reduced pressure, wherein the conditions of reduced pressure drying are a vacuum degree of 50 mbar and a temperature of 40 ° C to obtain the black saxifrage extract Mixed sample 70.8g;

[0050] Step 2, online free radical scavenger component screening: Add 5 times the mass of methanol with a volume concentration of 90% to 1 g of black saxifrage mixed sample to dissolve, prepare...

Embodiment 2

[0058] The separation and preparation process of natural free radical scavenger in black saxifrage, the method specifically includes the following steps:

[0059] Step 1, extraction: take 500g of whole saxifrage grass and dry it in the shade, coarsely crush it and extract it with methanol at a solid-to-liquid ratio of 1g: 100mL, extract twice at room temperature for 4 hours each time, filter and combine the filtrates, that is, filtrate A, The filtrate A is mixed according to the amount of polyamide: the amount of black saxifrage medicinal material = 1:0.5 and then dried under reduced pressure, wherein the conditions of reduced pressure drying are vacuum degree of 250 mbar and temperature of 60°C to obtain black saxifrage extract Material mixing sample 294.2g;

[0060]Step 2, online free radical scavenger component screening: add 10 times the mass of methanol with a volume concentration of 70% to 1 g of black saxifrage mixed sample to dissolve, prepare the sample concentration ...

Embodiment 3

[0068] The separation and preparation process of natural free radical scavenger in black saxifrage, the method specifically includes the following steps:

[0069] Step 1, extraction: take 1.2kg of whole saxifrage grass and dry it in the shade, coarsely crush it and extract it according to the ratio of material to liquid: 1g: 50mL of methanol, extract 3 times at room temperature, each time for 3 hours, filter and combine the filtrates, namely filtrate A , the filtrate A is mixed according to the amount of polyamide: the amount of black saxifrage medicinal material=1:0.5 and then dried under reduced pressure, wherein the conditions of reduced pressure drying are vacuum degree of 150 mbar and temperature of 50°C, to obtain black saxifrage Extract mixed sample 707.5g;

[0070] Step 2, online free radical scavenger component screening: Add 8 times the mass of methanol with a volume concentration of 80% to 1 g of black saxifrage mixed sample for dissolution, the prepared sample conc...

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Abstract

The invention relates to the technical field of separation of a natural free radical scavenger in Saxifraga atrata Engl, in particular to a separation preparation process and application of the natural free radical scavenger in Saxifraga atrata Engl. The preparation process comprises the following seven steps: extraction, online free radical scavenger component screening, microporous resin columncoarse separation, online free radical scavenger screening, reversed-phase preparative column preparation, reversed-phase preparative liquid chromatography purification of components Fr4-1 and Fr4-2,and hydrophilic preparative liquid chromatography purification of components Fr4-3 and Fr4-4. The method is low in cost, and the product purity is greater than 95%; the technical means adopted by theinvention can be used for large-scale production: the raw material requirement is not high, the cost is low, and batch preparation is easy; methanol room-temperature cold leaching extraction is adopted, and operation is easy; a microporous resin column is adopted for coarse separation, and the microporous resin separation material can be arranged in a medium-pressure column chromatography system and is easy for large-scale production; a reversed-phase preparative liquid chromatography or a hydrophilic preparative liquid chromatography used in separation and purification is a rapid isocratic method.

Description

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Claims

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Application Information

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Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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