Clonal line tea seedling tissue culture method

Abstract

The invention discloses a clonal line tea seedling tissue culture method, which comprises the following steps of: from June to August in the first year, selecting a female parent branch, and shearingthe thick and strong bud ear of the female parent branch to obtain a female ear; then, dipping the female ear in a root growing nutrient solution (which contains 20-30 g / L of mashed bananas, 0.06-0.18 g / L of 6-benzylaminopurine, 1.5-3 mg / L of brassinolide, 0.05-0.1 mg / L of diethylaminoethyl hexanoate and an MS culture medium) to carry out root growing culture; after white young roots grow fromthe female ear in the root growing nutrient solution and the white young roots are mutually twined, carrying out root division (i.e., a single female ear on which the white young roots grow is transplanted into a nutrient bag full of nutrient media, the lower part of the nutrient bag is provided with holes used for draining water and absorbing soil substances, and the nutrient bag is made of degradable materials), and then, carrying out indoor constant-temperature placement to a second year, and then, carrying out transplantation; and in March in the second year, transplanting a root divisionplant into a tea garden.

Description

technical field [0001] The invention relates to the technical field of tea plant breeding, in particular to a method for tissue culture of cloned tea seedlings. Background technique [0002] Clonal tea seedlings are bred from the branches of the female parent tea tree, maintaining good female parent characteristics, with high yield, high quality, neat germination, germination period of buds and leaves, growth, size of buds and leaves, color, hair, internode length Consistent and other characteristics, easy to pick and process, beneficial to the management of tea gardens and increase the yield of tea gardens, so the tissue culture method of clonal tea tree breeding has been widely recognized in recent years. [0003] The conventional method of tissue culture and planting of clone tea seedlings is as follows: from September to October of that year, the thick female branches are selected to cut buds and ears for centralized cutting seedlings, and the mother ears are rested in t...

AI Technical Summary

Problems solved by technology

It needs to be covered with film to keep warm in winter. When the temperature is below zero, it needs to be covered with double-layer film to keep warm. If the soil is frozen, it needs to be re-pressed after freezing to make it fully contact with the soil; the nursery needs to dig deep trenches to prevent water accumulation to prevent seedlings. The roots are rotten; it is necessary to keep the nursery soil moist at all times to ensure that the seedlings keep enough water to not lose the nutrients of the seedlings themselves. Rooting, but if it is too barren, it will lead to a longer growth cycle, so it is necessary to apply fertilizers frequently and thinly during the period from May to September of the next year. It is necessary to ensure the fertility needs of the growth of seedlings, and not to cause root burning due to excess fertility. In addition, due to the early growth of seedlings, young roots cannot compete with weeds for fertility and growth space in the summer of the following year, and it is necessary to manually pull out weeds to prevent them from being damaged. Long growth cycle leads to slow growth of tea gardens

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