Breeding method for high-outcrossing-rate high-combining-ability dual-purpose genic sterile lines

A technology of sterility and combining ability, applied in the fields of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of inability to observe, inability to express inferior traits, and low breeding efficiency, so as to improve the early selection efficiency. , the effect of improving outcrossing characteristics and seed commodity quality, and reducing production costs

Inactive Publication Date: 2020-05-19
YUAN LONGPING HIGH TECH AGRI CO LTD +2
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AI Technical Summary

Problems solved by technology

The dual-purpose GMS line is planted in the season in Changsha, and it is itself sterile, so it is impossible to observe the seed quality of a single plant of the CMS line to be selected. However, under the low temperature in Hainan, the seeds have good clover, good filling, and poor quality traits cannot be expressed. Low breeding efficiency of CMS lines with high seed commercial quality

Method used

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  • Breeding method for high-outcrossing-rate high-combining-ability dual-purpose genic sterile lines
  • Breeding method for high-outcrossing-rate high-combining-ability dual-purpose genic sterile lines
  • Breeding method for high-outcrossing-rate high-combining-ability dual-purpose genic sterile lines

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Experimental program
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Embodiment 1

[0045] Embodiment 1 Cultivation of rice dual-purpose GMS line Longke 638S

[0046] 1) The dual-purpose GMS line Xiangling 628S was used as the female parent to cross with C815S to obtain F 1 Generation seeds, the number of the hybrid combination is 3058S.

[0047] 2) Plant 7 plants of F 1 , remove false hybrids, F 1 Under natural low temperature or cold irrigation conditions, self-fertilization is strong, and the amount of seeds required for the next generation is harvested;

[0048] 3) Put the F of 3058S in Changsha, Hunan 2 Qunqun and Huazhan are planted at a ratio of 7:1. f 2 Group cutting 1500 plants, F 2 The population is planted in large double rows with a large plant spacing of 40cm and a small plant spacing of 20cm; according to 1 row restorer line - 3 double row F 2 -1 line of restoration -3 double lines of F 2 -……- 1 line restores the order of the line F 2 and restoration system, the schematic diagram of the overall planting plan is shown in figure 1 , for ...

Embodiment 2

[0057] Example 2 Cultivation of Rice Dual-purpose GMS Line Jing 4155S

[0058] Carry out this embodiment with reference to the mode of embodiment 1, and concrete process is briefly described as follows:

[0059] The dual-purpose GMS line Xiangling 628S was used as the female parent to cross with Y58S, and the combination number was 4155S. f 1 Under natural low temperature or cold irrigation conditions, self-fertilization is strong, and the amount of seeds required for harvesting the next generation is obtained. The F of 4155S 2 Qunqun and Huazhan are planted according to the ratio of 4155S: Huazhan = 7:1, primary selection based on agronomic characteristics, excellent sterile single plants, regeneration by cutting pockets, cold irrigation propagation, F 3 After the above natural outcross seeds are mature, the plants and rows are mixed and harvested, and the series 4155S / Huazhan's F are obtained 1 Seeds, planting identification advantages and rice quality, and using the "nat...

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Abstract

The present invention relates to the technical field of rice breeding, and specifically discloses a breeding method for high-outcrossing-rate high-combining-ability dual-purpose genic sterile lines. The method comprises the following steps of hybridizing two or more parents which have different genetic backgrounds and contain at least one two-line sterile line; collecting F2-generation seeds according to single plants; adjacently planting F2 generation and restorer lines sown by stages in the north in summer, selecting excellent sterile single plants according to breeding objectives of high outcrossing rate, high seed quality and the like after earing for rice root cutting regeneration, performing cold irrigation propagation, and collecting F3-generation seeds according to the single plants; planting F3 in the south in winter, performing natural seed setting by nature chilling, selecting the single plants with the low sterility critical temperatures and the good reproductivity, and collecting F4-generation seeds according to the plant rows; and continuing one-year north-south two-generation breeding until the sterile lines are stable, performing variety comparison on hybrid seeds matched with the sterile lines, and selecting the sterile lines corresponding to the hybrid seeds as the high-outcrossing-rate high-combining-ability dual-purpose genic sterile lines obtained by breeding. The method improves the probability of breeding high-outcrossing-rate high-combining-ability rice.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for breeding a dual-purpose nuclear sterile line with high outcrossing rate and high combining ability. Background technique [0002] Two-line hybrid rice has become an indispensable variety type in rice production, but there are still some problems to be solved (Chen Liyun et al. Thoughts and actions on some problems in the research and application of two-line hybrid rice. Chinese Rice Science, 2010, 24(6):641-646), breeding high-outcrossing rate, high combining ability, high-quality dual-purpose GMS lines has always been the main goal of hybrid rice breeding. [0003] The high cost of hybrid rice seed production is the main limiting factor for the large-scale application of hybrid rice. The two-line male sterile line was planted in Changsha in summer. Since the sensitive period of fertility conversion is in the high temperature season, it is completely sterile. ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 杨远柱符辰建符星学吴亚先孙毛山邓冰
Owner YUAN LONGPING HIGH TECH AGRI CO LTD
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