Fluorescent quantitative internal reference genes and their primers and applications in different tissues of Cinnamomum camphorensis

Abstract

The invention discloses a fluorescent quantitative internal reference gene of different tissues of Cinnamomum camphora and its primers and application, and belongs to the field of forestry insect molecular biology. The fluorescent quantitative internal reference genes are RPL18 and RPS15 genes, the nucleotide sequences of which are shown in SEQ ID NO.1 and SEQ ID NO.7. The present invention selects 8 candidate internal reference genes that are commonly used as internal reference genes through the transcriptome sequencing database of Cinnamomum chinensis, evaluates the stability of candidate internal reference genes through 5 algorithms, and obtains the fluorescence of different tissues of Cinnamomum syringus Quantify the internal reference gene, and design the real-time fluorescent quantitative PCR primers of the internal reference gene, which have strong specificity and high amplification efficiency. This application fills in the fact that there are no internal reference genes in different tissues of Cinnamomum chinensis, and provides a reliable analysis basis for the quantification of the expression of related functional genes in different tissue conditions of Cinnamomum syringus.

Description

technical field [0001] The invention belongs to the field of molecular biology of forestry insects, and in particular relates to fluorescent quantitative internal reference genes of different tissues of Cinnamomum camphorensis, primers and applications thereof. Background technique [0002] Pagiophloeus tsushimanus (Pagiophloeus tsushimanus) belongs to Coleptera of Coleptera, Curculionidae of Weevil family, and Pagiophloeus genus Pagiophloeus is a new record species in China. The adults mainly feed on the twig epidermis of 1-2 years old, and the larvae feed on the cambium between the xylem and the phloem, and the severely damaged camphor grows weak or dies. At present, Cinnamomum sinensis is only distributed in Shanghai, China. The insect has one generation per year in Shanghai, and overwinters as larvae and adults. At present, camphor forests in various ecological public welfare forests in Shanghai are affected by the insect in large areas, and there is a tendency to spre...

AI Technical Summary

Problems solved by technology

Therefore, it is necessary to screen the stability of internal reference genes in different tissues of the pest, otherwise it is impossible to quantitatively study the relative expression of the functional genes of the pest

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