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A kind of pulsatilla extract and its use in the preparation of prevention and/or treatment of cow mastitis

A technology of dairy cow mastitis and pulsatilla, which is applied in the field of medicine, can solve the problems of antibiotic residues, affecting the treatment effect, polluting milk sources, etc., and achieve the effect of treating dairy cow udder ulcer, shortening the healing time, and increasing milk production

Active Publication Date: 2021-09-17
广西英路维特药物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of antibiotics is likely to produce drug resistance and affect the treatment effect; at the same time, when antibiotics are used for treatment, it is easy to cause antibiotic residues, pollute milk sources, and endanger human health
It is worth noting that if mastitis is infected by Gram-negative bacteria, the use of lactam antibiotics will destroy the cell wall and release endotoxin, causing redness, swelling, heat, and pain in the entire breast area, which will aggravate the condition

Method used

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  • A kind of pulsatilla extract and its use in the preparation of prevention and/or treatment of cow mastitis
  • A kind of pulsatilla extract and its use in the preparation of prevention and/or treatment of cow mastitis
  • A kind of pulsatilla extract and its use in the preparation of prevention and/or treatment of cow mastitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 pulsatilla extract

[0030] 1. Pretreatment of medicinal materials

[0031] Take Pulsatilla Radix medicinal material, remove impurities, wash, cut into thin slices, and dry at 70°C until the water content is ≤13.0%, to obtain Pulsatilla Radix decoction pieces.

[0032] 2. Extraction and purification

[0033] (1) Take decoction pieces of Pulsatillae Radix, add 10 times the amount (mass volume ratio) of 70% ethanol to reflux and extract for 2-3 times, each time for 2-3 hours, to obtain the extract.

[0034] (2) Get the extract obtained in step (1), carry out gradient elution through AB-8 macroporous resin, and the eluent is water (2 times of resin volume), 20% ethanol (3 times of resin volume), 70% ethanol successively (3 times the resin volume), 95% ethanol (2 times the resin volume), collect the liquid eluted from 70% ethanol;

[0035] (3) The liquid collected in step (2) is decompressed to reclaim ethanol, then through medium-pressure...

Embodiment 2

[0040] Example 2 Preparation of Pulsatilla Radix Extract Injection

[0041] Weigh 5 g of Pulsatillae extract prepared in Example 1, add 800 mL of water for injection, dissolve it completely, filter, adjust the pH to 7.0, add water for injection to 1000 mL, fine filter, fill, seal, and sterilize at 100°C for 30 minutes, namely Obtain 5 mg / mL Pulsatilla extract injection.

experiment example 1

[0050] Experimental Example 1 Therapeutic Effects of Pulsatillae Extract on Cow Mastitis

[0051] (1) Test objects: 30 cases of patients with clinical cow mastitis. Among them, 15 were severely ill samples (symptoms: ulceration, no milk production). There were 15 mildly ill samples (symptoms: redness, swelling, less milk and turbidity).

[0052] (2) Test sample: the Pulsatillae extract injection of 5 mg / mL prepared in Example 2 of the present invention; the Pulsatilla saponin B4 injection of 5 mg / mL prepared in Comparative Example 1 and Comparative Example 2 and the Pulsatilla saponin of 5 mg / mL B5 injection.

[0053] (3) Administration method: use the injection to be tested to carry out local intramuscular injection to the affected breast area, once a day in the morning and in the evening, once a day in the morning and in the evening, each time, the dosage is 30ml / head, for 10 consecutive days.

[0054] (4) Evaluation criteria

[0055] Cure standards for mildly ill sample...

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Abstract

The invention provides a Pulsatillae extract and its use in the preparation of prevention and / or treatment of cow mastitis, and specifically discloses a Pulsatilla extract, in which the total content of Pulsatilla saponin B4 and Pulsatilla saponin B5 The content is greater than 80wt.%, and the ratio of Pulsatilla saponin B4 to Pulsatilla saponin B5 is (2.50‑4.50):1. Experimental results show that the Pulsatillae extract of the present invention can treat red and swollen dairy cow udders, treat cow udder ulceration, and increase milk production of dairy cows; the Pulsatilla saponin extract of the present invention can obviously shorten the average healing time of dairy cow mastitis, and improve the recovery time of dairy cow mastitis. The cure rate, its therapeutic effect on mastitis in dairy cows is significantly better than that of Pulsatilla saponin B4 or Pulsatilla saponin B5 alone, which has played a synergistic role in the preparation of prevention and / or treatment of viral and / or bacterial diseases, especially It has a very good application prospect in the medicine of cow mastitis.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a pulsatilla extract and its use in the preparation of preventing and / or treating cow mastitis. Background technique [0002] Dairy cow mastitis is one of the three major diseases of dairy cows recognized in the world. It occurs frequently during lactation. The main cause of its occurrence is pathogenic microorganism infection, in addition to improper milking operations, mechanical damage caused by trauma, and chemical reagent damage. Mastitis not only affects milk production and causes economic losses, but also affects milk quality and endangers people's health. [0003] Cow mastitis can be divided into subclinical mastitis and clinical mastitis two categories. Clinical mastitis can be seen with obvious clinical symptoms and visible changes in breast milk, and can be divided into three grades according to the degree of incidence: grade 1 mastitis: abnormal milk; grade 2 mast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61K31/704A61P15/14A61P31/04A61P31/12
CPCA61K31/704A61K36/71A61K2236/333A61K2236/39A61K2236/55A61P15/14A61P31/04A61P31/12
Inventor 苏柘僮赵俊卿蓝志杰杨世林
Owner 广西英路维特药物有限公司
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