Detection method for detecting adipogenic potential of mesenchymal stem cells

Abstract

A detection method for detecting adipogenic potential of mesenchymal stem cells comprises the following steps: respectively collecting d0 cells and d7 cells, and respectively extracting total RNA of the d0 cells and the d7 cells; synthesizing cDNA from the total RNA of the d0 cell and the d7 cell respectively; and detecting and calculating the relative expression quantity of the peroxisome proliferative activation receptor gamma 2 of the d0 cells and d7 cells by taking peroxisome proliferative activation receptor gamma 2 as a primer and 18S rRNA as an internal reference, and judging that the mesenchymal stem cells have the large adipogenesis potential when the ratio of X7:X0 is larger than or equal to 10. The detection method for detecting the adipogenesis potential of the mesenchymal stemcells can judge that whether the mesenchymal stem cells have large adipogenesis potential or not by detecting the relative expression quantity of the peroxisome proliferative activation receptor gamma 2 on the seventh day of induction, so the detection method has great significance in the adipogenesis induction culture process of the mesenchymal stem cells.

Description

technical field [0001] The invention relates to the technical field of cell detection, in particular to a detection method for detecting the adipogenic potential of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of adult stem cells with high self-renewal ability and multi-directional differentiation potential, widely present in bone marrow, fat, amniotic membrane, placenta, umbilical cord blood and umbilical cord tissue It can be induced to differentiate into various cells and tissues such as bone, fat, cartilage, nerve and cardiac muscle. [0003] Adipose tissue participates in the performance of physiological functions such as energy metabolism, organ protection and plasticity in the body. Clinical surgery has long used autologous fat for cosmetic and plastic treatment of the face, chest, buttocks and other parts. However, such operations require autologous surgical fat extraction, which leads to tissue trauma, pain and rela...

AI Technical Summary

Problems solved by technology

[0004] However, in the induction culture of the existing mesenchymal stem cells directed to adipocytes, the culture period is usually as long as more than 20 days

If the induced differentiation of mesenchymal stem cells fails, the culture time and culture cost will be excessively increased, so it is necessary to provide a detection method that can detect the adipogenic potential of mesenchymal stem cells during the directed induction culture process

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