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Serum-based method for rapid identification of Brucella vaccine strain infection and field strain infection

A technology for Brucella and vaccine strains, which is applied in the field of protein spectrum detection, can solve the problems that have not been used to identify Brucella vaccine strain infection and natural wild strain infection

Active Publication Date: 2020-06-23
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports at home and abroad on the use of MALDI-TOF MS technology to directly detect serum and confirm Brucella infection, and there is no report on the identification of Brucella vaccine strain infection and natural wild strain infection

Method used

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  • Serum-based method for rapid identification of Brucella vaccine strain infection and field strain infection
  • Serum-based method for rapid identification of Brucella vaccine strain infection and field strain infection
  • Serum-based method for rapid identification of Brucella vaccine strain infection and field strain infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Determination of characteristic proteins and construction of standard detection models

[0039] 1. Modeling training sample selection

[0040] The present invention adopts the serum sample of 30 routine Brucella wild strain infection patients, the serum sample of 30 routine Brucella vaccine strain infections, the vaccine strain of Brucella has S2 and A19, all Brucella vaccine strains Infected samples and Brucella wild strain infected samples were confirmed by epidemiological investigation and cysteine ​​agglutination test. 20% of the sample size is used for model cross-validation.

[0041] 2. Enrichment of serum protein samples

[0042] Take out the magnetic bead kit (Bruker MB-WCX kit) from the refrigerator at 4°C, take out a tube of weak cationic magnetic bead suspension, turn it upside down manually, and mix the magnetic bead suspension completely for 1 minute; draw 60 μL of magnetic bead binding buffer ( BB) into a 200 μL sample tube, then add 10 μL of ...

Embodiment 2

[0055] Example 2 Model Classification Ability Test

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Abstract

The invention provides a rapid identification method for Brucella vaccine strain infection and wild strain infection based on serum. According to the mass spectrometry data of human serum infected with Brucella vaccine strains and human serum infected with wild strains, the present invention constructs serum samples infected with Brucella vaccine strains and wild strains through the supervised neural network algorithm (SNN) model algorithm of ClinProTools software. The standard detection model was used, and the characteristic protein combination for distinguishing Brucella vaccine strains and wild strain infection sera was obtained. The recognition ability of the constructed model was 100%, and the cross-validation value was 95.13%. Using the standard detection model to classify and analyze the MALDI-TOF MS mass spectrometry data of the serum to be tested can accurately determine whether the serum to be tested is infected with a Brucella vaccine strain or a wild strain. The method of the invention has high accuracy, good repeatability, high flux, low detection cost, reliable result and good application prospect.

Description

technical field [0001] The invention relates to the technical field of protein spectrum detection, in particular to a standard detection model for detecting serum characteristic proteins and a method for identifying human serum infected with Brucella vaccine strains and wild strains based on the standard detection model. Background technique [0002] Brucellosis (brucellosis, referred to as brucellosis) is an infectious-allergic infectious disease caused by bacteria of the genus Brucella invading the body. Human infection is mainly through contact with infected animals or eating food contaminated by Brucella. After a person suffers from this disease, multiple organs may be damaged, and it is easy to turn into a chronic patient that is difficult to cure. In severe cases, it may become disabled and seriously affect the quality of life. [0003] Animal immunization is the most economical means to prevent and control animal brucellosis, but the antibodies produced after immuniz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G16B5/00G16B50/00
CPCG01N33/6851G01N33/6854G16B5/00G16B50/00G01N2333/23G01N2469/20
Inventor 肖迪姜海张炳华张慧芳王磊杨文涛赵飞李天一
Owner ICDC CHINA CDC
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