LC-MS/MS detection method for catecholamine and pretreatment kit

Abstract

The invention belongs to the technical field of catecholamine detection pretreatment, and particularly relates to an LC-MS / MS detection method for catecholamine and a pretreatment kit. The content ofcatecholamine in blood is reduced, and catecholamine is very easily oxidized, the half-life period is only 12h under a 4DEG C dark condition, and the requirement of high-throughput detection is hardlymet. The invention provides the LC-MS / MS detection method for catecholamine and the pretreatment kit, by optimizing the extraction method and adding a stabilizer, the extraction effect and stabilization effect on catecholamines in a blood sample are improved, so that the catecholamines can be kept stable within 24h after the sample is pretreated, and the requirement of high-throughput detection is met.

Description

technical field [0001] The invention belongs to the technical field of catecholamine detection and pretreatment, and in particular relates to a catecholamine LC-MS / MS detection method and a pretreatment kit. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Catecholamines (Catecholamines, CAs) are a class of amine compounds containing dihydroxyphenyl groups. They are very important neurotransmitters secreted by the adrenal medulla and some sympathetic neuron chromaffin cells, and are also important hormone substances. It mainly includes epinephrine (Epinephrine, E), norepinephrine (Norepinephrine, NE), and dopamine (Dopamine, DA). [0004] As neurotransmitters and hormones...

AI Technical Summary

Problems solved by technology

When using high-performance liquid chromatography tandem mass spectrometry for high-throughput detection, the amount of detection samples is large. Assuming that each sample detection takes 10 minutes, the detection of 60 samples takes at least 10 hours, which results in the sequence of detection and sorting of the extracted samples. Sequence causes poor accuracy in detecting samples

Moreover, the content of catecholamines in the human body is very low, and the loss during the extraction process and the stability of the sample after extraction have become difficult problems in the application of LC-MS / MS to the detection of catecholamines in human blood samples.

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