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Extraction method of radix asteris polysaccharide and radix asteris polypeptide

An extraction method, the technology of Aster, is applied in the field of extraction of Aster polysaccharides and Aster polypeptides, which can solve the problems of good extraction effect, high yield, and incomplete utilization, and achieve the goals of streamlining production links, improving the utilization of whole fruits, and saving time Effect

Inactive Publication Date: 2020-04-07
河北中科同创科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are many types of active ingredients in Aster, they have not been fully utilized at present, and there is a lack of comprehensive utilization methods.
For the extraction of active ingredients polysaccharides and polypeptides in Aster, there is no method with good extraction effect and high yield

Method used

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  • Extraction method of radix asteris polysaccharide and radix asteris polypeptide
  • Extraction method of radix asteris polysaccharide and radix asteris polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method for simultaneously extracting small molecular peptides and small molecular polysaccharides from asters comprises the following steps:

[0034] (1) Pretreatment of aster: After cleaning the aster with clean water, take 50 g of the aster, add water 5 times the mass of the aster, and perform beating treatment by mechanical method; and then ultrasonicate for 15 minutes under the condition of 20 kHz.

[0035] (2) Enzymolysis: add cellulase to the slurry, the mass ratio of cellulase to aster is 1:3000, adjust the pH to 5.5 at the same time, and enzymolysis time is 2 hours to obtain the first enzymolysis solution.

[0036] (3) Heat treatment: Pour the first enzymolysis solution into a container and heat for 30 minutes, set the temperature at 40°C, and cool for 12 hours after heating.

[0037] (4) Centrifugal treatment: put the heat-treated first enzymatic hydrolysis solution into a high-speed refrigerated centrifuge and centrifuge for 20 minutes with a c...

Embodiment 2

[0043] The preparation method for simultaneously extracting small molecular peptides and small molecular polysaccharides from Aster provided by this example includes the following steps:

[0044] (1) Pretreatment of aster: After cleaning the aster with clean water, take 50 g of the aster, add water 10 times the mass of the aster, and perform beating treatment by mechanical method; and then ultrasonicate for 35 minutes under the condition of 30 kHz.

[0045] (2) Enzymolysis: add cellulase to the slurry, the mass ratio of cellulase to aster is 1:4000, adjust the pH to 5.5 at the same time, and the enzymolysis time is 3 hours to obtain the first enzymolysis solution.

[0046] (3) Heat treatment: Pour the first enzymolysis solution into a container and heat for 40 minutes, set the temperature at 30°C, and cool for 12 hours after heating.

[0047] (4) Centrifugal treatment: put the heat-treated first enzymatic hydrolysis solution into a high-speed refrigerated centrifuge and centri...

Embodiment 3

[0053] The preparation method for simultaneously extracting small molecular peptides and small molecular polysaccharides from Aster provided by this example includes the following steps:

[0054] (1) Pretreatment of aster: After cleaning the aster with clean water, add water 15 times the mass of the aster, and perform beating treatment by mechanical method; and then ultrasonicate for 35 minutes under the condition of 20 kHz.

[0055] (2) Enzymolysis: add cellulase to the slurry, the mass ratio of cellulase to aster is 1:5000, adjust the pH to 6.5 at the same time, and the enzymolysis time is 3 hours to obtain the first enzymolysis solution.

[0056] (3) Heat treatment: Pour the first enzymolysis solution into a container and heat for 50 minutes, set the temperature at 40°C, and cool for 12 hours after heating.

[0057] (4) Centrifugal treatment: put the heat-treated first enzymatic hydrolysis solution into a high-speed refrigerated centrifuge and centrifuge for 25 minutes with...

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Abstract

The invention, which belongs to the technical field of plant extraction, provides an extraction method of radix asteris polysaccharide and radix asteris polypeptide. The method comprises the followingsteps: step one, mixing and pulping radix asteris and water, and carrying out ultrasonic treatment to obtain a radix asteris slurry; step two, mixing the radix asteris slurry with cellulase, and carrying out first enzymolysis; step three, treating the first enzymatic hydrolysate at a temperature of 30-50 DEG C for 30-50 minutes, and then carrying out solid-liquid separation; step four, adjustingthe pH value of first clear liquid to be 4-7, mixing the first clear liquid with a sulfate mixture, and carrying out salting-out layering to obtain a second supernatant and lower-layer precipitation liquid; step five, mixing the lower-layer precipitation solution with alkaline protease, carrying out second enzymolysis to obtain a second enzymatic hydrolysate, and performing ultrafiltration and nanofiltration on the second enzymatic hydrolysate and drying the processed solution to obtain radix asteris polypeptide; and step six, drying the second supernatant obtained in the step four to obtain the radix asteris polysaccharide. With the method, simultaneous extraction of the radix asteris polysaccharide and the radix asteris polypeptide can be realized; and the yield is high.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting aster polysaccharides and aster polypeptides. Background technique [0002] Aster is the dried root and rhizome of Aster (Aster tataricus L.), a plant of the genus Aster in the family Asteraceae. , mainly produced in Anguo, Hebei and Bozhou, Anhui. Aster has the effect of nourishing the lungs, reducing phlegm and relieving cough, and it is mainly used to treat symptoms such as dyspnea and cough with excessive phlegm, persistent cough, and overworked cough. The active ingredients in Aster are very rich, mainly including terpenes and their glycosides, peptides, sterols, flavonoids, anthraquinones, coumarins, small molecule polysaccharides, etc., among which triterpene saponins are the main characteristic of Aster Composition, cyclic peptide is a kind of characteristic chemical composition of Aster, and it is the main component of Aster...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/30C07K1/34C08B37/00
CPCC07K1/145C07K1/303C07K1/34C08B37/0003C12P21/06
Inventor 郭强贺佩贾志军
Owner 河北中科同创科技发展有限公司
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