Method of inducing Chinese rose flowering in test tube

Abstract

The invention belongs to the technical field of plant tissue culture, and relates to a method of inducing Chinese rose flowering in a test tube. The method includes the following steps: sterilizing the explant of Chinese rose to obtain a sterile explant; inoculating the sterile explant in a first MS culture medium to induce callus, wherein the first MS culture medium contains 0.1-10mg / L of 6-BA, 0.1-2mg / L of NAA, 4-10g / L of agar and 20-50g / L of sucrose; inoculating the callus into a second MS culture medium for differentiation and culture to obtain a young stem, wherein the second MS culture medium contains 0.01-2mg / L of 6-BA, 0.1-2mg / L of NAA, 4-10g / L of agar and 20-50g / L of sucrose; and inoculating the young stem into a third MS culture medium with alternating photoperiod and dark cyclefor rooting and flowering culture, wherein the third MS culture medium contains 0.1-10mg / L of IBA, 0.1-2mg / L of NAA, 0.01-1mg / L of iron nanoparticles, 4-10g / L of agar and 20-50g / L of sucrose, with thepH value being 3-7. The method can induce Chinese rose flowering in the test tube.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for inducing rose flowering in a test tube. Background technique [0002] Rose belongs to Rosaceae, Rosa genus, rose species (Latin name Rosa chinensis Jacq.). Rose is an important flower plant and one of the four major cut flowers in the world. It is native to China and has a wide distribution in the world. Chinese rose flowering in four seasons can be used as an ornamental plant, but also as a medicinal plant. At the same time, rose is an important experimental material in biological science research. Realizing the flowering of rose in test tube through tissue culture method can provide a shortcut for rose bioscience research. To provide a theoretical basis for further exploring the mechanism of plant flowering. [0003] The existing rose tissue culture is mainly to adjust the differentiation and proliferation ability of rose tissue to ...

AI Technical Summary

Problems solved by technology

The rose tissue culture method in the prior art is mainly used for the rapid propagation of fine varieties and the basic operation of biotechnology in rose transgenics, but there is no research and development of the tissue culture method for roses to bloom and bear fruit in test tubes, because roses bloom and bear fruit in test tubes Different from flowering and fruiting in the soil, the test tube is a relatively closed microenvironment controlled by humans. The difficulty lies in the control of light, temperature, and the ratio of nutrients and hormones in the medium to meet the needs of the rose in the test tube.