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A method of exogenous ga3 inducing rose flowering

A rose and exogenous technology, applied in the field of cell engineering, can solve the problems of lack of good quality of rose, failure to meet aesthetic requirements, and decline of varieties, and achieve the effects of shortening the flowering cycle, increasing ornamental value, and long flowering period

Inactive Publication Date: 2018-11-20
JILIN NORMAL UNIV
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Problems solved by technology

In the process of traditional breeding at home and abroad, most scientists only focus on the improvement of the apparent traits of roses, such as the shape, diameter, and quantity of flowers, while ignoring the retention of excellent qualities such as survival rate, lifespan, and tolerance. The lack of good quality of a variety of roses, resulting in the decline of varieties
Therefore, traditional hybrid breeding methods are far from meeting people's increasing aesthetic requirements.

Method used

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  • A method of exogenous ga3 inducing rose flowering
  • A method of exogenous ga3 inducing rose flowering
  • A method of exogenous ga3 inducing rose flowering

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Embodiment 1

[0024] The MS medium used in the specific embodiment of the present invention is formulated with reference to the MS medium formulation method described in Chinese patent CN 104115743B. Moreover, all culture media were sterilized by high-pressure steam at 121°C for 20 minutes after preparation, and the culture media were condensed to room temperature before use.

[0025] 1), the acquisition of rose aseptic vaccine

[0026] a. Buy ordinary roses at the flower market, about 20cm-40cm high, pink in color, cut off the strong branches of roses in the same year, remove the dust on the surface with a soft brush, put them in a beaker filled with clean water, add a small amount of cleaning agent (1- 3 drops of white cat detergent) soaked for 10 minutes, during which the beaker was shaken continuously, rinsed with running water for 1 hour, and sterilized with Cl2 for 20 minutes; in the ultra-clean workbench, soaked in sterile distilled water for 3 times, and blotted dry with sterile fil...

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Abstract

The invention discloses an exogenous GA3 induced Chinese rose flowering method. According to the method, a certain concentration of exogenous GA3 is applied to a culture medium in the Chinese rose flower bud induction process by adopting a plant tissue culture technique to induce Chinese rose flowering, and a specific formula of the culture medium adopts an MS culture medium, saccharose, agar and gibberellin (GA3). The concentration of the saccharose is 40g / L, the concentration of the agar is 7.2 g / L, the concentration of the GA3 is 100mg / mL, and the pH is 5.83-5.85. Finally, aseptic Chinese rose seedlings good in growth state, bright in color and luster and high in ornamental value are successfully cultivated in the flowering cultivation process, the flowering rate is improved, and a blooming period of Chinese roses is greatly shortened.

Description

technical field [0001] The invention belongs to the technical field of cell engineering, and relates to a method for inducing rose flowering by exogenous GA3, in particular to a method for inducing flowering of aseptic rose seedlings by exogenous GA3. Background technique [0002] Rose (Rosahybrida) has a history of more than 1,000 years in my country. It is mainly divided into six categories: species rose, strong flower rose, vine rose, perfume rose, shrub rose and rose. During the three years from 2005 to 2008, a total of 831 rose varieties were registered internationally, including 169 Fenghua roses, 204 hybrid perfume roses, 89 small flower roses, 161 shrub roses, and 106 roses. In foreign countries, rose was introduced to Britain and France in the 17th and 18th centuries. After that, European people used it as an important germplasm to crossbreed with rose, and cultivated many high-quality new varieties. At present, more than 20,000 new rose varieties have been bred a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 周晓馥宁思淇武慧
Owner JILIN NORMAL UNIV
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