Diffraction super-resolution Microscopic imaging method and system

Abstract

The invention relates to a microscopic imaging method and system. According to the microscopic imaging method, array light spots (larger than or equal to two) are generated by utilizing a diffractionsuper-resolution element, wherein the array light spots are imaged on an object plane of the microscopic imaging system so as to realize illumination of an imaged object, and then the imaging efficiency is improved. The imaged object is scanned by utilizing the array light spots, image information of the scanned imaged object is obtained, and image reconstruction is carried out according to the image information. According to the microscopic imaging method, the diffraction super-resolution element is utilized to modulate an illumination light beam to generate the super-resolution array light spots, the diffraction limit of an optical system can be broken through, optical super-resolution imaging is realized, and the super-resolution capability is adjustable. t

Description

technical field [0001] The present application relates to the field of microscope imaging, in particular to a diffraction super-resolution microscopic imaging method and system. Background technique [0002] Compared with the electron microscope, the optical microscope can perform real-time dynamic fast imaging of biological samples under physiological conditions, and has become a vital research tool for life science researchers. However, due to the existence of the optical diffraction limit, the highest resolution of optical microscopy is limited to around half a wavelength, which seriously hinders life science researchers from conducting finer research on the sub-wavelength scale. Breaking through the optical diffraction limit has become a core problem that needs to be solved urgently in the fields of ultra-precision machining, ultra-precision detection and life science research. Various super-resolution techniques such as confocal laser scanning microscopy (CLSM), stimul...

AI Technical Summary

Problems solved by technology

[0003] Among the above super-resolution technologies, CLSM uses single-point scanning objects to achieve super-resolution reconstruction, but the super-resolution capability is limited and uncontrollable; SIM uses structured light to load high-frequency information into images, and obtains super-resolution images after reconstruction. Resolve images, but are limited by the field of view and the resolution is doubled at most; STED and STORM use the difference between the stimulated emission of fluorescent molecules and the light wave of autofluorescence to compress the point spread function, rather than directly improving the optical system Obtain a focused spot that breaks through the diffraction limit

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