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A method for solid-state fermenting tobacco stem cut stems with Rhododendron jelly

A technology of solid-state fermentation and Rhodotorula japonicus, which is applied in the fields of application, tobacco, and tobacco treatment, and can solve problems such as environmental pollution, high cost, and chemical reagent pollution

Active Publication Date: 2021-09-14
CHINA TOBACCO SICHUAN IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for treating tobacco stems mainly include physical blasting, chemical treatment, and biological enzyme methods, which have disadvantages such as poor degradation effect, chemical reagent pollution, and high cost.
At present, it is reported that Bacillus pumilus V35 is used to make a bacterial agent for the treatment of cut stems, but it only degrades cellulose and pectin, and its cell wall component lignin is not degraded, so the degradation effect of its cell wall substances is not obvious
At present, the utilization rate of tobacco stems is low, and its large backlog will not only cause environmental pollution, but also cause waste of available resources

Method used

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  • A method for solid-state fermenting tobacco stem cut stems with Rhododendron jelly
  • A method for solid-state fermenting tobacco stem cut stems with Rhododendron jelly
  • A method for solid-state fermenting tobacco stem cut stems with Rhododendron jelly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Slant culture: inoculate Rhodotorula gelatin strains on the slant medium (i.e. YEPD medium), and cultivate at 30°C for 2 days;

[0048] (2) Liquid seed culture: Inoculate the slant strain into a 250mL Erlenmeyer flask with 80mL seed medium, and cultivate it for 3 days at 30°C;

[0049] (3) solid-state fermentation culture: the cultured Rhodotorula gluei seed liquid is inoculated into the 500mL Erlenmeyer flask of the fermentation medium of tobacco stem shreds by 10% of the inoculum size, cultivated for 6 days at 30°C, Before fermentation, the contents of cellulose, hemicellulose, lignin and pectin were 585.4mg / g, 167.18mg / g, 96mg / g and 66.40mg / g respectively, and the contents of reducing sugar and total sugar were 17.25%, 18.36% %. After 6 days of fermentation, the measured cellulose degradation rate was 20.69%, the hemicellulose degradation rate was 40.15%, the lignin degradation rate was 14.46%, the pectin degradation rate was 13.20%, the reducing sugar increased...

Embodiment 2

[0053] The cultivated rhodotorula gum seed solution was inoculated into the 500mL Erlenmeyer flask of the fermentation medium of cut tobacco stems according to the inoculum size of 20%, and other conditions were the same as in Example 1, and the cellulose degradation was measured after the fermentation rate 32.81%, hemicellulose degradation rate 33.53%, lignin degradation rate 14.07%, pectin degradation rate 11.78%, reducing sugar content increased 30.55%, total sugar increased 24.51% (see table 2, figure 1 and figure 2 ).

[0054] When table 2 inoculum size is 20%, the substance content change that different culture time detects

[0055]

Embodiment 3

[0057] The cultivated Rhodotorula gluei seed solution was inoculated into the 500mL Erlenmeyer flask of the fermentation medium of cut tobacco stems by 30% of the inoculum size, and other conditions were the same as in Example 1, and the cellulose degradation was measured after the fermentation was finished. rate 25.68%, hemicellulose degradation rate 28.32%, lignin degradation rate 17.50%, pectin degradation rate 8.91%, reducing sugar content increased 27.13%, total sugar content increased 27.34% (seeing table 3, figure 1 and figure 2 ).

[0058] When the inoculum size of table 3 was 30%, the change of substance content detected in different culture time

[0059]

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Abstract

The invention discloses a method for solid-state fermentation of shredded tobacco stems by using Rhodotorula gum. The method of the invention uses Rhodotorula gum as a fermentation strain to carry out solid-state fermentation of shredded tobacco stems, which is mainly realized through the following steps: (1) Inclined surface cultivation (2) seed culture; (3) solid-state fermentation culture, inoculate the cultivated seed liquid into the solid medium containing tobacco stem shreds, and cultivate it for 2-6 days at 30°C, the tobacco stem of the present invention passes through the glue red Yeast fermentation and biotransformation, in which the tobacco stem cell wall material is decomposed and utilized by Rhodotorula gluei, which increases the content of reducing sugar and total sugar, and improves the utilization rate of tobacco stem in cigarettes.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for solid-state fermentation of shredded tobacco stems by using Rhodotorula gum. Background technique [0002] As a big tobacco country, my country ranks first in the world in terms of planting area and output of tobacco, and a large amount of tobacco stems are also produced therefrom. At present, the methods for processing tobacco stems mainly include physical blasting, chemical treatment, and biological enzyme methods, which have disadvantages such as poor degradation effect, chemical reagent pollution, and high cost. At present, it is reported that Bacillus pumilus V35 is used to make a bacterial agent for the treatment of cut stems, but it only degrades cellulose and pectin, and its cell wall component lignin is not degraded, so the degradation effect of its cell wall substances is not obvious. At present, the utilization rate of tobacco stems is low,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B5/16A24B15/20
CPCA24B5/16A24B15/20
Inventor 李东亮张娟刘文莉丁重阳朱鹏程陈坚堵国成
Owner CHINA TOBACCO SICHUAN IND CO LTD
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