Rhododendron dauricum culture medium and plant regeneration method

A culture medium and basic medium technology, applied in plant regeneration, botany equipment and methods, horticultural methods, etc., can solve the problems of long breeding cycle of Rhododendron xingan seeds, inability to maintain excellent traits of parents, low rooting rate, etc., and achieve easy The effect of inducing regeneration plants, not easy to die, and high rooting rate

Pending Publication Date: 2020-02-04
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of long breeding cycle of Rhododendron xing'an seeds, inability to maintain good traits of parents, low rooting rate and easy death of asexual cutting method propagation, and to provide a culture medium and plant regeneration method for Rhododendron xing'an

Method used

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  • Rhododendron dauricum culture medium and plant regeneration method
  • Rhododendron dauricum culture medium and plant regeneration method
  • Rhododendron dauricum culture medium and plant regeneration method

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specific Embodiment approach 1

[0030] Specific embodiment one: In this embodiment, the Xing'an rhododendron basal culture medium includes macroelements, moderate elements, trace elements, organic elements, sucrose and agar, with a pH value of 5.0 to 5.4;

[0031] The bulk element is NH 4 NO 3 350mg / L~450mg / L, (NH 4 ) 2 SO 4 120mg / L~150mg / L, KNO 3 150mg / L~250mg / L, KH 2 PO 4 350mg / L~450mg / L, MgSO 4 ·7H 2 O 350mg / L~400mg / L and CaCl 2 2H 2 O 420mg / L~480mg / L;

[0032] The middle element is Na 2 -EDTA 35mg / L~40, FeSO 4 ·7H 2 O 25mg / L~30mg / L, H 3 BO 4 5mg / L~8mg / L, MnSO 4 4H 2 O 15mg / L~20mg / L and ZnSO 4 ·7H 2 O 5mg / L~10mg / L;

[0033] The trace element is Na 2 MoO 4 2H 2 O 0.1mg / L~5mg / L, CuSO 4 ·5H 2 O 0.01mg / L~0.05mg / L and CoCl 2 ·6H 2 O 0.01mg / L~0.05mg / L;

[0034] The organic elements are VB1 0.2mg / L-0.5mg / L and 50mg / L-150mg / L inositol;

[0035] The sucrose is 25g / L-30g / L, and the agar is 7g / L-8g / L.

specific Embodiment approach 2

[0036] Specific embodiment two: In this embodiment, Rhododendron xingan initiation differentiation medium is composed of rhododendron xingan basal medium, gibberellin and indole butyric acid described in specific embodiment one, and gibberellin in every liter of rhododendron xingan initiation differentiation medium 1mg~2.0mg, indole butyric acid 0.2mg~1mg.

specific Embodiment approach 3

[0037] Specific embodiment three: In this embodiment, the culture medium for Rhododendron xing'an multiplication and strong seedlings is composed of Rhododendron xingan basal medium, gibberellin, and indole butyric acid in specific embodiment one. 0.5mg~1mg, indole butyric acid 0.5mg~1.5mg.

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Abstract

The invention relates to a plant culture medium and a plant regeneration method, in particular to a rhododendron dauricum culture medium and a plant regeneration method. The problems of long propagation period of rhododendron dauricum seeds, incapability of retaining parents' excellent traits, low rooting rate in asexual cuttage propagation and high proneness to death are solved. The rhododendrondauricum culture medium comprises macroelements, medium elements, microelements, organic elements, cane sugar, agar and water, and the pH value ranges from 5.0 to 5.4. A rhododendron dauricum start and differentiation medium, a rhododendron dauricum multiplying and seedling strengthening medium and a rhododendron dauricum rooting medium comprise rhododendron dauricum base media, gibberellin and indolebutyric acid respectively. The culture medium is used for regeneration and cultivation of rhododendron dauricum plants, the differentiation cycle is 25-30 days, the inducing rate for cluster seedlings exceeds 85%, the rooting rate is high, and the rhododendron dauricum is not liable to die.

Description

technical field [0001] The invention relates to a plant culture medium and a plant regeneration method. Background technique [0002] First of all, due to long-term cross-pollination of woody plants, the plants have high heterozygosity, which affects the continuation of their excellent traits. The use of seeds for propagation cannot completely maintain the excellent traits of the parents, and the offspring will have traits segregated. If conventional methods are used for genetic improvement, it takes five to six generations of selfing to obtain a pure line, which takes a long time and is limited by the season, so the seedling rate is low; High, very easy to die due to dehydration, so the offspring produced by natural reproduction cannot meet the market demand, seriously hindering the promotion and development of this excellent tree species. Third, the rooting rate of Rhododendron xing'an asexual cutting method is extremely low, and the roots of cuttings are easy to brown an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/04
CPCA01G7/045A01H4/001A01H4/008Y02P60/14
Inventor 王丹王福德马盈舒钰赵学丽温爱亭王钰婷姜璟瑜刘霞杨扬马静
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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