Saccharomycete Q7-7 from deep sea, and application thereof

A yeast, deep-sea technology, applied in applications, fungi, microorganism-based methods, etc., can solve problems such as lack of obvious effect

Active Publication Date: 2020-01-31
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When amylase is added alone in the feed, its effect is not obvious

Method used

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  • Saccharomycete Q7-7 from deep sea, and application thereof
  • Saccharomycete Q7-7 from deep sea, and application thereof
  • Saccharomycete Q7-7 from deep sea, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Isolation and identification of strains

[0039] A strain of Septoria sp. was isolated from oceanic sediment samples, which can produce amylase, lipase and protease at the same time, and can be used to prepare yeast feed additives.

[0040]The yeast Q7-7 derived from the deep sea is Sporidiobolus pararoseus Q7-7, which was deposited in the China Center for Type Culture Collection on August 26, 2019, address: Wuhan University, Wuhan, China, postcode : 430072, and the preservation number of the depository center is CCTCC NO: M 2019662.

[0041] The microbiological characteristics of the deep sea-derived yeast Q7-7 are as follows: after being cultured on a YPD plate at 28° C. for 72 hours, the front side of the colony is orange-red, and the back side is orange-pink. The surface of the colony is uneven, the gloss is dark, and the edge is irregular, and the color of the center of the colony is consistent with that of the edge. The surface is viscous and easy to provoke....

Embodiment 2

[0058] The optimal preparation of the ITS sequence fermentation medium of the yeast Q7-7 of embodiment 2 deep-sea origin

[0059] The present invention selects a variety of carbon sources, nitrogen sources and other growth factors that can be used in the yeast Q7-7 fermentation medium. At 28°C, the liquid volume is ml / 250m, the inoculum volume is 1%, and the rotation speed is 180r / min. Yeast Q7-7 fermentation culture comparison was carried out under the culture conditions, and the most suitable medium for yeast Y5 fermentation was selected by weighing after fermentation to 72 hours after comprehensive consideration of indicators such as bacterial content and enzymes. Optimizing fermentation medium usually adopts single factor method and orthogonal experiment design method, and single factor analysis of variance is a simple analysis of variance model. It only needs to separate the factor effect from the error effect, and judge the significance of the factor effect by comparing ...

Embodiment 3

[0073] The optimization and growth curve of embodiment 3 yeast Q7-7 fermentation conditions

[0074] The optimization of the fermentation process plays a very important role in the fermentation industry, especially in the fermentation production, it is a very necessary technical means to improve the fermentation index. There are many methods to optimize the fermentation process. They are not isolated but interrelated. They are often a combination of multiple optimization methods. Microorganisms may be affected by environmental conditions in different stages of growth and in different stages of producing target metabolites. There are different requirements. Therefore, the temperature, pH value, dissolved oxygen, stirring speed, etc. should be constantly changed in the bioreactor to provide the best environmental conditions for it, and finally the maximum biomass and optimal metabolites can be obtained.

[0075] The microbial growth curve is a curve drawn with the number of mic...

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Abstract

The invention relates to saccharomycete Q7-7 from the deep sea, and application thereof, and relates to an aquatic feed. The saccharomycete Q7-7 from the deep sea is (Sporidiobolus pararoseus) Q7-7 which is preserved in the China Center For Type Culture Collection on August 26th, 2019, and has the preservation number being CCTCC NO: M2019662. The saccharomycete Q7-7 from the deep sea can be applied in preparing a novel aquatic feed additive. A deep-sea yeast separated from an ocean sample is amplified by using ITS4 and ITS5 primers so as to obtain an ITS sequence, the favorable growth vigour is found in the culture process, amylase, lipase and protease can be produced, and a fermentation liquor mixture can be produced to the aquatic feed additive. Through a four-factor three-level orthogonal test including temperature, rotary speed, inoculum size and loading volume, the best fermentation condition is determined, and the application is determined through a shrimp seedling culture experiment.

Description

technical field [0001] The invention relates to aquatic feed, in particular to a strain of yeast Q7-7 producing amylase, lipase and protease derived from deep sea and its application. Background technique [0002] Aquafeed is bait specially provided for aquatic animal breeding. According to feeding species, aquatic feed can be divided into fish feed, shrimp feed and crab feed, etc.; according to feed characteristics, it can be divided into compound feed, concentrated feed and premixed feed. The raw materials for the production of aquatic feed are mainly composed of fishmeal, grain raw materials and oil, and fishmeal and grain raw materials often account for more than 50% of the feed cost. In recent years, with the rapid development of the aquaculture industry, feed additives with higher benefits have been developed and applied one after another, and have achieved good benefits. The use of green and environmentally friendly aquatic feed additives is attracting more and more...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N15/10A23K10/18A23K50/80C12R1/645
CPCA23K10/18A23K50/80C12N15/1003C12N1/145C12R2001/645
Inventor 汤熙翔王起林冯莹
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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