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SERS sensor based on Au-Se interface for quantitative detection of ultra-sensitive and high-fidelity biological molecules

A sensor, au-se technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve problems such as high-fidelity quantitative detection of low-content molecules, and achieve universal applicability, simple operation methods, and easy large-scale production Effect

Inactive Publication Date: 2020-01-24
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the use of Au-Se bonds instead of Au-S bonds to construct nanoprobes has been applied to bioluminescent analysis, but it still cannot solve the in vivo high-fidelity quantitative detection of low-content molecules.

Method used

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  • SERS sensor based on Au-Se interface for quantitative detection of ultra-sensitive and high-fidelity biological molecules
  • SERS sensor based on Au-Se interface for quantitative detection of ultra-sensitive and high-fidelity biological molecules
  • SERS sensor based on Au-Se interface for quantitative detection of ultra-sensitive and high-fidelity biological molecules

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Embodiment 1

[0041] A new type of bonding method forms a more stable Au-Se SERS sensor, which can provide a new strategy for highly sensitive and high-fidelity detection of biomolecules in complex physiological systems.

[0042] (1) Synthesis and characterization of the probe

[0043] First, fresh AuNPs were prepared by sodium citrate reduction method (1g of chloroauric acid was fixed in a 100mL volumetric flask to obtain a chloroauric acid solution with a mass fraction of 1%, and the mass fraction of 1mL was accurately taken with a pipette gun. Add 1% chloroauric acid into a brown volumetric flask, and then settle to 100mL with ultrapure water. We weighed 1.13037g of sodium citrate when preparing a 1% sodium citrate solution Solid, add it to a 100mL volumetric flask, then add ultrapure water and ultrasonically dissolve it completely, and shake it well. Then fix the 100mL three-necked flask above the intelligent magnetic stirrer, and add magnets to the three-necked flask, pay attention to ...

Embodiment 2

[0050] A SERS sensor with a core-shell structure modified with an internal standard is used for the absolute quantitative detection of living cells (lung adenocarcinoma cell A549).

[0051] (1) Synthesis and characterization of core-shell nanoparticles In order to further realize high-fidelity and absolute quantitative detection in cells and in vivo, a core-shell nanomaterial with internal standard molecules was constructed, and the gap between the core-shell nanoparticles was modified with internal standard 1 , 4-BDT molecule, can protect the internal standard molecule from the physiological environment, the core-shell nanoparticle synthesis process such as Figure 5 As shown in A, the specific preparation method is as follows: (a) synthesis of gold core. Gold cores are synthesized by the seed growth method. First by vigorously mixing 4.5 mL of water, 5 mL of CTAC solution (0.2 M), 450 μL of NaBH 4 solution (0.02M) and 515 μL HAuCl 4 (4.86mM) to prepare the seed solution. ...

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Abstract

The invention relates to a SERS sensor based on an Au-Se interface for quantitative detection of ultra-sensitive and high-fidelity biological molecules. The TAMRA-modified SeH-polypeptide chain is assembled on the surface of AuNPs through Au-Se. Due to the surface plasmon resonance effect, TAMRA exhibits a strong Raman signal, the peptide chain is cleaved by activated MMP-2 when MMP-2 is present,and TAMRA signaling molecules are far away from AuNPs, which leads to the reduction of Raman signals. Compared with Au-S Raman nanoprobes, Au-Se SERS sensor has better resistance to biothiol interference. The invention uses a new type of bonding method to form a more stable Au-Se SERS sensor, which can provide a new strategy for high-sensitivity and high-fidelity detection of biomolecules in complex physiological systems. A SERS sensor with a core-shell structure modified with an internal standard can be used for absolute quantitative detection of living cells.

Description

technical field [0001] The invention belongs to the technical field of biological detection and Raman detection, and in particular relates to a SERS sensor based on an Au-Se interface for ultrasensitive high-fidelity biomolecular quantitative detection. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The accurate quantification of biomolecules in complex biological systems is an important issue of common concern in the fields of biology and analytical chemistry. Due to the low content of many biomolecules in organisms, the low sensitivity of commonly used detection methods has become a bottleneck for the detection of low content molecules. In addition, the complex physio...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 唐波李璐李晓晓段小艳
Owner SHANDONG NORMAL UNIV
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