Method for diagnosing bovine subacute rumen acidosis by using urinary calcium, urine pH and excrement acid detergent fibers

An acid washing fiber and ruminal acidosis technology, which is applied in material inspection products, instruments, analytical materials, etc., can solve the problems of economic loss in the breeding industry, easy secondary laminitis, and increased mortality rate, and reduce ranch economy. effect of loss

Pending Publication Date: 2020-01-17
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

With the transformation of my country's dairy cattle and beef cattle industry from the traditional scattered breeding model to a large-scale, intensive and standardized breeding model, as well as the promotion of high-yield breeding selection and one-sided pursuit of high yield and other factors, the current large-scale cattle farms have a high incidence of important common diseases. , causing huge economic losses to the farming industry
Subacute rumen acidosis is a common nutritional metabolic disease in lactating cows and fattening beef cattle. The disease leads to reduced milk production of cows, low fattening benefits of beef cattle, increased mortality rate, and prone to secondary laminitis. caused serious economic losses
At present, the gold standard for the diagnosis of subacute rumen acidosis is still to puncture the rumen and test the pH of the rumen fluid. However, the above-mentioned diagnostic methods are very likely to cause rumen damage in cattle and cause stress in the herd, which is not conducive to the detection of herd diseases in the herd, resulting in subacute herd disease. Difficult Diagnosis of Acute Rumen Acidosis

Method used

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  • Method for diagnosing bovine subacute rumen acidosis by using urinary calcium, urine pH and excrement acid detergent fibers
  • Method for diagnosing bovine subacute rumen acidosis by using urinary calcium, urine pH and excrement acid detergent fibers
  • Method for diagnosing bovine subacute rumen acidosis by using urinary calcium, urine pH and excrement acid detergent fibers

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Embodiment

[0009] Animal experiments: 2-3 parities were randomly selected in an intensive dairy farm in Heilongjiang Province, fed with TMR (Total Mixed Rations) and with an annual milk production of 11.5 tons, 7-14 days after delivery. 44 Holstein dairy cows were divided into subacute rumen acidosis group (5.26.2, without any other symptoms) according to the pH value of rumen fluid 6 hours after eating. Among them, 24 were in the healthy group, and 20 were in the subacute rumen acidosis group. The urine and feces of the experimental cows were collected.

[0010] Feces: 200g of fresh feces were collected from the rectum. The samples were mainly used to detect dry matter (105°C), acid detergent fiber (ADF), crude protein, crude fat, and starch. All detection indicators were analyzed and detected by near-infrared method.

[0011] Urine: 50ml of urine in the middle of urination was collected by vaginal massage, and urine Ca, creatinine (CREA), urea (BUN), uric acid (UA), urine protein, and...

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Abstract

The invention provides a method for diagnosing subacute rumen acidosis of dairy cows by using blood ALT, blood L lactic acid, milk fat and milk urea. The blood ALT, the blood L lactic acid, the milk fat and the milk urea are sensitive to subacute rumen acidosis of dairy cows, thus subacute rumen acidosis of dairy cows is diagnosed through detecting content of the blood ALT, the blood L lactic acid, the milk fat and the milk urea, and the content of the blood ALT, the blood L lactic acid, the milk fat is extremely and significantly correlated with the milk urea and the subacute rumen acidosis of dairy cows. The method is characterized by determining that: the sensitivity for diagnosing the dairy cows suffering from subacute rumen acidosis is 100.00% and the specificity is 57.14% when the blood ALT content is less than 16 U/L; the sensitivity for diagnosing the dairy cows suffering from subacute rumen acidosis is 75.00% and the specificity is 90.48% when the blood L lactic acid content is greater than 0.37 mmol/L; the sensitivity for diagnosing the dairy cows suffering from subacute rumen acidosis is 50.00% and the specificity is 100.00% when the milk fat content is less than 3.41%;and the sensitivity for diagnosing the dairy cows suffering from subacute rumen acidosis is 84.62% and the specificity is 90.48% when the milk urea content is less than 0.02%.

Description

Technical field: [0001] The invention relates to a method for diagnosing bovine subacute rumen acidosis, in particular to a method for diagnosing bovine subacute rumen acidosis by using urine calcium, urine pH and feces acidic detergent fiber. Background technique: [0002] As one of the important pillar industries of the agricultural and rural economy, animal husbandry is an important starting point for realizing "rural revitalization" and "poverty alleviation". It is related to agricultural and rural farmers such as food security, food safety, energy conservation and emission reduction, farmer employment and international trade It is the basic and strategic demand to lead China's agricultural and rural areas to realize modernization and sustainable development. With the transformation of my country's dairy cattle and beef cattle industry from the traditional scattered breeding model to a large-scale, intensive and standardized breeding model, as well as the promotion of hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N33/48G16B40/00G16C20/70
CPCG01N21/359G01N33/48G16B40/00G16C20/70
Inventor 杨威徐闯吕鑫泉张冰冰夏成张洪友王爽赵莹莹麻芯茹高飞王凭波刘桂池
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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