High-accuracy microbial detection chip

Abstract

The invention provides a high-accuracy microbial detection chip. Through arranging sidewalls of microreaction chambers as hydrophilic surfaces, forms of sample liquid droplets in the microreaction chambers are changed, and the sample liquid droplets can have flattened oil-water interfaces, so that the degree of convergence or divergence of radiated light at the oil-water interfaces is lowered, then, the problem of mutual interference of fluorescence signals of adjacent microreaction chambers is weakened, and a fluorescent detection result can be more accurate; and further, incomplete gas exhausting due to the fact that oily filling liquid difficultly enters the microreaction chambers during gas exhausting and a bubble optical false appearance caused by the incomplete gas exhausting are prevented. The invention further provides a microreaction chamber sidewall with changeable surface properties, by virtue of a ring electrode and a hydrophobic coating wrapping the outside of the ring electrode, surface characteristics of the microreaction chamber sidewall can be selectively adjusted according to an on-off state between the ring electrode and a round point electrode, so that the microreaction chamber sidewall is allowed to form good wetting with the oily filling liquid during gas exhausting and form good wetting with the aqueous sample liquid droplets during sample introduction.

Description

technical field [0001] The present application relates to a microfluidic chip. Specifically, it relates to a high-precision PCR detection chip capable of reducing optical interference. Background technique [0002] The PCR chip is currently an important means for microbial detection, which can realize rapid amplification, labeling and quantitative detection of nucleic acids through various functional units integrated on a chip. At present, the mainstream PCR chip detection methods need to rely on optical means in the quantification step, such as fluorescent markers and CCD cameras to obtain specific reaction results. Existing PCR chips have been able to achieve relatively accurate quantitative detection, such as digital PCR detection methods. However, precise does not mean accurate. The PCR chip usually has an arrayed micro-reaction chamber, and samples are distributed in the arrayed micro-reaction chamber in the form of micro-droplets. By irradiating excitation light to ...

AI Technical Summary

Problems solved by technology

In this process, when the fluorescent signal from the microsphere reaches the spherical interface, due to the difference in the refractive index of the two media, there will be a change in the direction of propagation, such as convergence or further divergence; and this phenomenon It will lead to the interference of the fluorescent signals of adjacent micro-reaction chambers, and even the fluorescent signals of two adjacent micro-reaction chambers are mixed into the light spots captured by the CCD camera, making it difficult to accurately distinguish the actual number of fluorescent signals

On the other hand, this change in the propagation direction of the radiated light may also cause the fluorescent signals emitted by some micro-reaction chambers in the entire micro-reaction chamber array to exceed the photosensitive range of the CCD camera and cannot be captured.

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