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Application of pearl oyster small molecule peptide in skin wound repair

A technology of small molecular peptides and wound repair, applied in the field of biomedicine, can solve the problems of underutilized shellfish meat, and achieve the effect of facilitating proliferation and repair, good biological characteristics, and good affinity

Pending Publication Date: 2019-12-31
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After pearl oysters are harvested, a large amount of shell meat has not been fully utilized

Method used

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  • Application of pearl oyster small molecule peptide in skin wound repair
  • Application of pearl oyster small molecule peptide in skin wound repair
  • Application of pearl oyster small molecule peptide in skin wound repair

Examples

Experimental program
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Effect test

Embodiment 1

[0060] An application of a pearl oyster small molecule peptide in wound repair, characterized in that the pearl oyster small molecule peptide has a new application for repairing skin wounds;

[0061] The preparation method of the pearl oyster small molecule peptide comprises the following steps:

[0062]S1. Preparation of Pearl Oyster Enzymolyzate;

[0063] The pearl oyster mantle is separated by pretreatment; the protease selected for enzymolysis of the pearl oyster mantle is animal protease, the amount of protease added is 4000U / g, the pH value is 7.5, the enzymolysis temperature is 50°C, the enzymolysis time is 4 hours, and 100°C After inactivating the enzyme for 10 minutes, centrifuge to get the supernatant;

[0064] S2. The supernatant obtained in step S1 is selected from a ceramic membrane with a pore size of 0.05-0.5um. After being fully filtered, ultrafiltration membranes with different molecular weight cut-offs are used to separate and collect peptides with different...

Embodiment 2

[0087] An application of a pearl oyster small molecule peptide in wound repair, characterized in that the pearl oyster small molecule peptide has a new purpose of repairing skin wounds;

[0088] The preparation method of the pearl oyster small molecule peptide comprises the following steps:

[0089] S1. Preparation of Pearl Oyster Enzymolyzate;

[0090] The pearl oyster mantle is separated by pretreatment; the protease selected for enzymolysis of the pearl oyster mantle is neutral protease, the amount of protease added is 3000U / g, the pH value is 6.5, the enzymolysis temperature is 45°C, and the enzymolysis time is 3 hours, 100 After inactivating the enzyme at ℃ for 10 minutes, centrifuge to get the supernatant;

[0091] S2. The supernatant obtained in step S1 is selected from a ceramic membrane with a pore size of 0.05-0.5um. After being fully filtered, ultrafiltration membranes with different molecular weight cut-offs are used to separate and collect peptides with different...

Embodiment 3

[0109] An application of a pearl oyster small molecule peptide in wound repair, characterized in that the pearl oyster small molecule peptide has a new application for repairing skin wounds;

[0110] The preparation method of the pearl oyster small molecule peptide comprises the following steps:

[0111] S1. Preparation of Pearl Oyster Enzymolyzate;

[0112] The pearl oyster mantle is separated by pretreatment; the protease selected for enzymolysis of the pearl oyster mantle is alkaline protease, the amount of protease added is 5000U / g, the pH value is 10, the enzymolysis temperature is 55°C, and the enzymolysis time is 5 hours, 100 After inactivating the enzyme at ℃ for 10 minutes, centrifuge to get the supernatant;

[0113] S2. The supernatant obtained in step S1 is selected from a ceramic membrane with a pore size of 0.05-0.5um. After being fully filtered, ultrafiltration membranes with different molecular weight cut-offs are used to separate and collect peptides with diff...

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Abstract

The invention provides an application of pearl oyster small molecule peptide in skin wound repair. The pearl oyster small molecule peptide has a new application of repairing skin wounds. The preparation method of the pearl oyster small molecule peptide comprises the following steps: S1, preparing a pearl oyster zymolyte; and S2, selecting a ceramic membrane with a pore diameter of 0.05-0.5 mu m, performing full filtration, separating the material by adopting ultrafiltration membranes with different molecular weight cutoff, collecting peptide fragments with different molecular weights, including any one of bioactive peptide with the molecular weight of less than 3000Da, bioactive peptide with the molecular weight of 3000Da-5000Da and bioactive peptide with the molecular weight of 5000Da-10000Da, and performing freeze-drying to obtain the pearl oyster small molecule peptide. The invention provides a new application of the pearl oyster small molecule peptide in preparing a medicine for repairing skin wounds such as skin burn, wound, burn and scald, the effect of the pearl oyster small molecule peptide is obviously superior to that of the medicine in the prior art, the wound is quicklyrepaired, no scar is left, and the effect of promoting wound repair is more obvious.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of a pearl oyster small molecule peptide in skin wound repair. Background technique [0002] Worldwide, more than 40 million people suffer from skin trauma each year. If the damaged skin cannot be repaired in time, the wound will be easily infected and become a chronic wound, severe trauma or even life-threatening. A good remodeling effect will reduce skin scars and even achieve scarless healing. [0003] Wound healing mainly includes four periods: hemostasis and coagulation, inflammation, proliferation and tissue remodeling. After the body suffers trauma, platelets are activated and secrete related factors to promote blood coagulation into thrombus to achieve coagulation and stimulate the body to produce inflammatory reactions. During the inflammatory phase, the immune system engulfs tissue fragments and kills environmental pollutants such as...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K38/39A61K9/70A61P17/02A61P7/04A61P31/04A61P29/00A61L15/22A61L15/42A61L15/44A61L15/46D04H1/728D04H1/4309D04H1/435D01D5/00C12P21/06C07K1/34A61K31/722
CPCA61K38/012A61K38/39A61K31/722A61K9/7007A61P17/02A61P7/04A61P31/04A61P29/00A61L15/225A61L15/42A61L15/425A61L15/44A61L15/46D04H1/728D04H1/4309D04H1/435D01D5/003D01D5/0092C12P21/06C07K1/34A61L2300/252A61L2300/41A61L2300/412A61L2300/404A61L2400/04A61L2300/236A61K2300/00C08L89/00C08L5/08
Inventor 林海生秦小明章超桦伍彬曹文红高加龙杨发明苏伟明郑惠娜
Owner GUANGDONG OCEAN UNIVERSITY
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