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Use of a derivative of asiatic acid for promoting the proliferation of human adipose-derived mesenchymal stem cells in vitro and maintaining stemness

A technology of asiatic acid and stem cells, applied in animal cells, vertebrate cells, microorganisms, etc., to achieve the effect of promoting in vitro proliferation and in vitro proliferation promotion

Active Publication Date: 2021-09-17
湖南南华生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] No asiatic acid and 2,3-dicarbonyl-23-carboxy n-butyl ester-ursane-12-ene-28-carboxylate ethyl ester, 2,3-dicarbonyl-23-carboxyisobutyl ester-urethane Effects of ethyl thane-12-ene-28-carboxylate on the proliferation and differentiation of ADSCs in vitro

Method used

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  • Use of a derivative of asiatic acid for promoting the proliferation of human adipose-derived mesenchymal stem cells in vitro and maintaining stemness
  • Use of a derivative of asiatic acid for promoting the proliferation of human adipose-derived mesenchymal stem cells in vitro and maintaining stemness
  • Use of a derivative of asiatic acid for promoting the proliferation of human adipose-derived mesenchymal stem cells in vitro and maintaining stemness

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Embodiment Construction

[0023] The following describes the substantive content of the present invention in detail, but does not limit the protection scope of the present invention.

[0024] 1. Experimental materials

[0025] High-glucose DMEM medium was purchased from Gibco, USA.

[0026] Fetal bovine serum was purchased from Gibco, USA.

[0027] Fluorescence-labeled monoclonal antibodies were purchased from Bioscience, USA.

[0028] Asiatic acid, 2,3-dicarbonyl-23-carboxy n-butyl ester-ursane-12-ene-28-carboxylate ethyl ester (derivative A), 2,3-dicarbonyl-23-carboxyisobutyl Ester-ursane-12-ene-28-carboxylate ethyl ester (derivative B) is purchased or self-made, with a purity of not less than 98%.

[0029] Toluidine blue dye kit was purchased from Solarbio.

[0030] Adipogenic induction medium of human adipose-derived mesenchymal stem cells was purchased from Saiye Biotech.

[0031] Human adipose-derived mesenchymal stem cell osteogenesis induction medium was purchased from Saiye Biotech.

[0...

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Abstract

The invention discloses the use of an asiatic acid derivative for promoting the proliferation of human adipose-derived mesenchymal stem cells in vitro and maintaining stemness. It was found that asiatic acid, 2,3-dicarbonyl-23-carboxy n-butyl ester-ursane-12-ene-28-carboxylate ethyl ester, 2,3-dicarbonyl-23-carboxy isobutyl ester- The effects of ethyl ursane‑12‑ene‑28‑carboxylate on human adipose-derived mesenchymal stem cells are common and different. All three can significantly promote the proliferation of human adipose-derived mesenchymal stem cells in vitro, but asiatic acid in While promoting the proliferation of human adipose-derived mesenchymal stem cells in vitro, it can promote its chondrogenic differentiation, which is suitable for the application of human adipose-derived mesenchymal stem cells in chondrogenic formation, while 2,3-dicarbonyl-23-carboxy n-butyl ester-ursane- Ethyl 12-ene-28-carboxylate and ethyl 2,3-dicarbonyl-23-carboxylate-ursane-12-ene-28-carboxylate can promote the proliferation of human adipose-derived mesenchymal stem cells in vitro While maintaining its stem cell characteristics, it is suitable for the rapid expansion of human adipose-derived mesenchymal stem cell seed cells in vitro.

Description

technical field [0001] The invention belongs to the field of biology, and relates to in vitro culture and expansion of adipose-derived mesenchymal stem cells, in particular to an application of asiatic acid derivatives for promoting in vitro proliferation of human adipose-derived mesenchymal stem cells and maintaining stemness. Background technique [0002] Adipose-derived mesenchymal stem cells (ADSCs) are a kind of stem cells with multi-directional differentiation potential isolated from adipose tissue, which can be induced to differentiate into fat, bone, cartilage, pancreatic β-cells and cardiac muscle under specific conditions. cell. [0003] In recent years, ADSCs have significantly increased each year in tissue repair and regeneration research. Researchers are most concerned about the number of ADSCs used in repair and regeneration, clinical trials evaluating efficacy and safety. According to the US Clinical Trials (2015) report, there are 121 studies registered usi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2500/30
Inventor 杨姣姣
Owner 湖南南华生物技术有限公司
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