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Rapid propagation method of taxus mairei

A southern yew, rapid propagation technology, applied in the field of plant propagation, can solve the problems of slow growth, achieve the effects of increasing survival rate and rooting rate, shortening propagation time, and reducing the occurrence of browning

Inactive Publication Date: 2019-12-13
JIANGSU HONGDOUSHAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its seeds have a dormancy period and must be treated with low-temperature stratification before they can emerge in the same year, and their growth is relatively slow. Therefore, it is of great significance to find a suitable method to promote the growth of Taxus chinensis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of rapid propagation method of southern yew, comprising the following steps:

[0017] (1) Select the 5-10-year-old southern yew plant as the mother plant, and cut it from the mother plant as an explant before the swelling scales of the buds of the year have not cracked;

[0018] (2) Disinfect the clipped explants with 75% alcohol for 30s on the ultra-clean workbench, pour off the alcohol, rinse with sterile water for 3-4 times, and then rinse with 0.1% HgCl 2 Disinfect for 5-15 minutes, and finally rinse with sterile water;

[0019] (3) Inoculate the washed explants on the bud induction medium and cultivate them for 15 days, the culture temperature is 24°C, the daily light time is 12 h, and the light intensity is 1800-2000 lx; ​​the bud induction medium is MS+ 0.4 mg / L NAA + 1.5 mg / L 6-BA + 1g / L activated carbon + 30 g / L sucrose + 7g / L agar;

[0020] (4) Take out the new shoots when they grow to 2-3cm, soak the base of the new shoots with a concentration of 50...

Embodiment 2

[0023] A kind of rapid propagation method of southern yew, comprising the following steps:

[0024] (1) Select the 5-10-year-old southern yew plant as the mother plant, and cut it from the mother plant as an explant before the swelling scales of the buds of the year have not cracked;

[0025] (2) Disinfect the clipped explants with 75% alcohol for 30s on the ultra-clean workbench, pour off the alcohol, rinse with sterile water for 3-4 times, and then rinse with 0.1% HgCl 2 Disinfect for 5-15 minutes, and finally rinse with sterile water;

[0026] (3) Inoculate the washed explants on the bud induction medium and cultivate them for 17 days at a culture temperature of 25°C, with a daily light time of 14 h and a light intensity of 1800-2000 lx; ​​the bud induction medium is MS+ 0.3 mg / L NAA + 1.3 mg / L 6-BA + 1g / L activated carbon + 30 g / L sucrose + 7g / L agar;

[0027] (4) Take out the new shoots when they grow to 2-3 cm, soak the base of the new shoots with a concentration of 5...

Embodiment 3

[0030] A kind of rapid propagation method of southern yew, comprising the following steps:

[0031] (1) Select the 5-10-year-old southern yew plant as the mother plant, and cut it from the mother plant as an explant before the swelling scales of the buds of the year have not cracked;

[0032] (2) Disinfect the clipped explants with 75% alcohol for 30s on the ultra-clean workbench, pour off the alcohol, rinse with sterile water for 3-4 times, and then rinse with 0.1% HgCl 2 Disinfect for 5-15 minutes, and finally rinse with sterile water;

[0033] (3) Inoculate the rinsed explants on the bud induction medium and cultivate them for 20 days, the culture temperature is 26°C, the daily light time is 15 h, and the light intensity is 1800-2000 lx; ​​the bud induction medium is MS+ 0.5 mg / L NAA + 1.7 mg / L 6-BA + 1g / L activated carbon + 30 g / L sucrose + 7g / L agar;

[0034] (4) Take out the new shoots when they grow to 2-3 cm, soak the base of the new shoots with 500 mg / L IBA solutio...

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Abstract

The invention relates to a plant propagation method, in particular to a rapid propagation method of taxus mairei. The method comprises the following steps of selecting an explant; sterilizing the explant; inducing buds, wherein a bud induction culture medium is an MS culture medium in which 0.3-0.5 mg / L of NAA, 1.3-1.7 mg / L of 6-BA, 1g / L of activated carbon, 30 g / L of sucrose and 7 g / L of agar areadded; performing root induction, wherein a rooting culture medium is a 1 / 2 MS culture medium in which 0.4-0.6 mg / L of KT, 1.8-2.2 mg / L of NAA, 90-110 MG / l of AgNO3, 1 g / L of activated carbon, 7 g / Lof agar and 30 g / L of sucrose are added; domesticating tissue culture seedlings. The method improves the survival rate and rooting rate of tissue culture, shortens the propagation time and improves the propagation efficiency.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of the southern yew. Background technique [0002] Southern yew is one of the unique components of subtropical to warm temperate regions in China. It is scattered in broad-leaved forests. Its wood is hard, its texture is dense, its image is beautiful, it does not warp or crack, and it has strong corrosion resistance. It can be used for construction, high-end furniture, and interior decoration. It is worth mentioning that its dry roots, branches and leaves, and bark all contain a taxane compound-paclitaxel, and the roots and leaves have the highest content. However, its seeds have a dormant period and must be treated with low-temperature stratification before they can emerge in the same year, and their growth is relatively slow. Therefore, it is of great significance to find a suitable method to promote the growth of Taxus chinensis. Contents of the invent...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 朱波风
Owner JIANGSU HONGDOUSHAN BIOLOGICAL TECH
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