A kind of transgenic rape and its product screening positive plasmid molecule pycsc-1905 and its application

Abstract

The invention discloses the design and application of a transgenic rapeseed and its product screening positive plasmid pYCSC-1905, and relates to the safety supervision and screening detection technology of the transgenic rapeseed in the field of transgenic safety. The base sequence of pYCSC‑1905 is shown in SEQ ID NO.1. Utilizing pYCSC‑1905 as a positive control and a quality control sample, ordinary PCR and real-time fluorescent PCR screening and detection of transgenic components can be performed on rapeseed and its products, and the coverage rate of transgenic rapeseed with known genetic information is 100%; the present invention It solves the technical problem of lack of positive controls or standard products in the screening of transgenic rapeseed, avoids the problem of setting multiple positive controls for multiple detection targets in the screening test, and reduces the labor cost and economic cost of preparing multiple positive controls .

Description

technical field [0001] The invention relates to the safety supervision, screening and detection technology of transgenic rape in the field of transgenic safety, in particular to the design and application of a positive plasmid molecule pYCSC-1905. Background technique [0002] Over the past 20 years, the global R&D and commercialization of GMOs have developed rapidly. Since 1992, 4,133 regulatory approvals for GMOs have been approved worldwide, involving 476 transformants of 26 GMOs, with a cumulative planting area of ​​more than 2.3 billion hectares. huge economic benefits. At the same time, the safety of genetically modified organisms has always attracted attention from all walks of life. For this reason, more than 50 countries and regions, including China, the European Union, the United States, Japan and South Korea, have promulgated and implemented laws and regulations on the safety management of genetically modified organisms. Standardize the research and development a...

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Problems solved by technology

[0006] After searching existing patents and other documents, no report has been found on the use of the design of the present invention or a similar design to construct a universal positive plasmid molecule and application for screening and detection of transgenic rapeseed

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