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Design for screening positive plasmid molecule pYCSC-1905 on transgenic oilseed rape and product thereof and application of design

A technology of pycsc-1905 and plasmid molecules, applied in application, plant products, genetic engineering, etc., can solve the problems of undiscovered transgenic rapeseed screening positive plasmid molecules and applications

Active Publication Date: 2019-12-06
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After searching existing patents and other documents, no report has been found on the use of the design of the present invention or a similar design to construct a universal positive plasmid molecule and application for screening and detection of transgenic rapeseed

Method used

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  • Design for screening positive plasmid molecule pYCSC-1905 on transgenic oilseed rape and product thereof and application of design
  • Design for screening positive plasmid molecule pYCSC-1905 on transgenic oilseed rape and product thereof and application of design
  • Design for screening positive plasmid molecule pYCSC-1905 on transgenic oilseed rape and product thereof and application of design

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Embodiment Construction

[0156] The following describes the present invention in detail with reference to the drawings and embodiments:

[0157] 1. Construction of the positive plasmid molecule pYCSC-1905

[0158] (1) Determine targets for genetically modified rape screening and detection

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Abstract

The invention discloses design for screening positive plasmid molecule pYCSC-1905 on transgenic oilseed rape and a product thereof and application of the design, and relates to a safety supervision and screening detection technology of the transgenic oilseed rape in the field of transgenic safety. The base sequence of the pYCSC-1905 is as shown in SEQ ID NO. 1. The pYCSC-1905 is used as a positivecontrol and quality control sample, common PCR and real-time fluorescent PCR screening detection of transgenic ingredients on the oilseed rape and product thereof can be carried out, and the coveragerate on transgenic oilseed rape with known genetic information at present is 100%; according to the invention, the technical problem of lack of positive controls or standard substances in transgenicoilseed rape screening detection is solved, the problem that multiple positive controls are set for multiple detection targets in screening detection is avoided, and the labor cost and economic cost for preparing multiple positive controls are reduced.

Description

Technical field [0001] The invention relates to the safety supervision and screening detection technology of genetically modified rape in the field of genetically modified safety, in particular to the design and application of a positive plasmid molecule pYCSC-1905. Background technique [0002] In the past 20 years, the global R&D and commercialization of genetically modified organisms has developed rapidly. Since 1992, 4133 regulatory approvals for genetically modified products have been approved globally, involving 476 transformants of 26 genetically modified crops, and the cumulative planting area has exceeded 2.3 billion hectares. Has a huge economic benefit. At the same time, the safety of genetically modified organisms has always been a concern from all walks of life. For this reason, more than 50 countries and regions, including China, the European Union, the United States, Japan, and South Korea, have promulgated and implemented laws and regulations on the safety managem...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00A01H6/20C12Q1/6895
CPCC12N15/8201C12Q1/6895C12Q2600/13C12Q2600/166
Inventor 武玉花张秀杰李俊李夏莹王灏潜翟杉杉陈子言张洪文高鸿飞李允静吴刚
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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