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Preparation method of liquid sample Raman spectrum detection, and equipment thereof

A Raman spectrum detection and liquid sample technology, applied in the field of spectrum detection, can solve the problems of high cost, poor repeatability, poor fixability and replaceability of aluminum foil and quartz glass slides, etc., and achieve the effect of low cost and stable experimental system

Pending Publication Date: 2019-12-03
INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, for the detection base of Raman spectrum detection of liquid samples, generally a layer of aluminum foil is covered on the quartz glass slide, and a drop of liquid sample is sampled, dried at room temperature and photographed. This method is not only costly, but also the aluminum foil and The fixation and replaceability of quartz glass slides is poor, the amount of liquid samples is not easy to control, and the repeatability is poor

Method used

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  • Preparation method of liquid sample Raman spectrum detection, and equipment thereof
  • Preparation method of liquid sample Raman spectrum detection, and equipment thereof
  • Preparation method of liquid sample Raman spectrum detection, and equipment thereof

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Experimental program
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Embodiment 1

[0032] A preparation method for liquid sample Raman spectrum detection of the present invention comprises the following steps:

[0033] 1. Use the flattened tinfoil 3 as the base to reduce the background background of Raman scattering, and fix the tinfoil 3 on the quartz glass slide 1;

[0034] 2. Take 5 microliters of liquid sample 4 and drop it on the tinfoil 3, spot the sample vertically, keep the ambient temperature at 25 degrees Celsius and let it stand for 30 minutes, and the liquid sample 4 will dry up within 30 minutes;

[0035] 3. Collect the Raman spectrum of the dried liquid sample;

[0036] 4. Replace the tinfoil, and repeat the operation according to the quantification, room temperature and resting time of the liquid sample 4 set in step 2.

Embodiment 2

[0038] A preparation method for liquid sample Raman spectrum detection of the present invention comprises the following steps:

[0039] 1. Use the flattened tinfoil 3 as the base to reduce the background background of Raman scattering, and fix the tinfoil 3 on the quartz glass slide 1;

[0040] 2. Take 4 microliters of liquid sample 4 and drop it on the tinfoil 3, spot the sample vertically, keep the ambient temperature at 26 degrees Celsius and let it stand for 20 minutes, and the liquid sample 4 will dry up within 20 minutes;

[0041] 3. Collect the Raman spectrum of the dried liquid sample;

[0042] 4. Replace the tinfoil, and repeat the operation according to the quantification, room temperature and resting time of the liquid sample 4 set in step 2.

Embodiment 3

[0044] A preparation method for liquid sample Raman spectrum detection of the present invention comprises the following steps:

[0045] 1. Use the flattened tinfoil 3 as the base to reduce the background background of Raman scattering, and fix the tinfoil 3 on the quartz glass slide 1;

[0046] 2. Take 10 microliters of liquid sample 4 drops on the tinfoil 3, spot the sample vertically, keep the ambient temperature at 24 degrees Celsius and let it stand for 60 minutes, the liquid sample 4 will dry up within 60 minutes;

[0047] 3. Collect the Raman spectrum of the dried liquid sample;

[0048] 4. Replace the tinfoil, and repeat the operation according to the quantification, room temperature and resting time of the liquid sample 4 set in step 2.

[0049] To sum up, under the set amount of liquid sample 4 taken, room temperature and resting time, by controlling these factors, repeated experiments of Raman scattering can be effectively carried out, which is beneficial to elimina...

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Abstract

The invention relates to the technology of the spectrum detection, specifically a preparation method of liquid sample Raman spectrum detection, and equipment thereof. The method comprises the following steps: one, reducing a base background by taking a flattened tin-foil paper as the base, and fixing the tin-foil paper on a quartz glide; two, dropping quantitative liquid sample on the tin-foil paper, performing vertical sample application, maintaining that the room temperature is located at the room temperature, and standing for the fixed time, wherein liquid sample is dried up in the fixed standing time; three, performing Raman spectrum collection on the dried liquid sample; and four, replacing the tin-foil paper, and repeating the operation according to the quantification, room temperature, and standing time of the liquid sample set in the step two. Through the preparation method disclosed by the invention, the aluminum foil can be successfully replaced, the sample quantity is controllable, and the experiment system is stable.

Description

technical field [0001] The invention relates to spectrum detection technology, in particular to a preparation method and equipment for Raman spectrum detection of liquid samples. Background technique [0002] Raman spectroscopy is a kind of scattering spectrum, the principle of which is that when light passes through a transparent medium and is scattered by molecules, the frequency will change, so that the scattering spectrum with a frequency different from that of the incident light can be analyzed to obtain molecular vibration and rotation information , and apply this method to molecular structure studies. Today, Raman spectroscopy is used in various fields such as chemistry, physics, biology and medicine, and is of great value for pure qualitative analysis, highly quantitative analysis and determination of molecular structure. [0003] However, the Raman spectrum itself is very weak, and the introduction of any substance will bring some degree of pollution to the measure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 白杨邱录贵于珍
Owner INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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