High-flux nucleic acid extraction system

Abstract

The invention belongs to the technical field of biology and particularly relates to a high-flux nucleic acid extraction system. The high-flux nucleic acid extraction system provided by the invention comprises an operating bin, a manipulator, a magnetic rod, a magnetic sleeve, a reaction device, an extraction device, a liquid distribution device, a sample code scanning device and a control device,wherein the manipulator comprises at least two third shafts capable of doing three-dimensional motion, and the extraction device is movably arranged on one third shaft and comprises a first loading component for fixing the magnetic rod and a second loading component for fixing the magnetic sleeve, so as to separately enable the magnetic rod to extend into the magnetic sleeve or shift out from themagnetic sleeve and enable the magnetic sleeve to extend into the reaction device or shift out from the reaction device; and the liquid distribution device is movably arranged on another third shaft and comprises at least two distributing needles, so as to separately switch over between two states, i.e., extending into the reaction device or shifting away from the reaction device. Under the actionof the control device, the manipulator is high in running accuracy and good in stability; and meanwhile, the sample code scanning device performs automated identification on samples, so that the efficiency of nucleic acid extraction is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-throughput nucleic acid extraction system. Background technique [0002] Nucleic acid is an important class of biological macromolecules that store and transmit life information, and is also the core molecule of modern biochemistry and molecular biology research. With the rapid development of molecular biology in recent years, a large number of nucleic acid-based molecular biology experiments have emerged, such as next-generation sequencing, fluorescent quantitative PCR, gene chips, nucleic acid molecular hybridization, etc., and the integrity of DNA, The degree of degradation of RNA and the purity and concentration of nucleic acid will have more or less influence on the results of molecular biology experiments. Therefore, the first problem facing molecular biology experiments is how to quickly and efficiently extract relatively pure DNA from complex samples. high...

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the cumbersome extraction steps, high error rate, and the presence of samples due to the insufficient degree of automation, poor coordination and flexibility of each functional module of the existing nucleic acid extractor based on the magnetic rod method. Contamination and other defects, thus providing a high-throughput nucleic acid extraction system with better flexibility and integration of each functional module, simple extraction steps, and low error rate

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