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Remediation method for pesticide-contaminated soil based on degradation by laccase

A technology for pesticide pollution and soil remediation, applied in the field of pesticide-contaminated soil remediation, can solve problems such as inactivation of laccase, and achieve the effects of reducing reaction time, short remediation period and high efficiency

Inactive Publication Date: 2019-11-26
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the adsorption or encapsulation of pollutants during the degradation process prevents the further interaction of laccases, which may be an important reason for the inactivation of laccases.

Method used

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  • Remediation method for pesticide-contaminated soil based on degradation by laccase
  • Remediation method for pesticide-contaminated soil based on degradation by laccase
  • Remediation method for pesticide-contaminated soil based on degradation by laccase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Use calcium oxide or acetic acid to adjust the pH value of the soil to be repaired to about 4.5, and then use a buffer solution with a phosphate concentration of 100mM to accurately adjust the pH to 4.5, add 0.2mg / g of HBT, and the enzyme addition amount is 0U / g 土 , 1U / g 土 , 2U / g 土 , 4U / g 土 , adjusted the water content to 15%, placed in a constant temperature incubator at 25°C for 48 hours, and measured the concentration of paclobutrazol / uniconazole at 6 time points of 0h, 3h, 6h, 12h, 24h, and 48h. Experimental results such as figure 1 As shown, paclobutrazol and uniconazole pesticides basically tended to be stable after being degraded for 24 hours. After 48 hours of the whole degradation, the degradation rates of paclobutrazol under different laccase concentrations were 10%, 59%, 36% and 66%, and the degradation rates of uniconazole were 7.7%, 45%, 42% and 44%, respectively. It is proved that under the above conditions, the concentration of laccase per gram of soi...

Embodiment 2

[0042] Use calcium oxide or acetic acid to adjust the pH of the soil to be repaired to about 4.5, then use a phosphate buffer with a concentration of 100mM to precisely adjust the pH to 4.5, and add laccase to form 8U / g 土 Add 0.2mg / g of HBT to keep the moisture content of the contaminated soil at 15%, and set different temperature conditions in batches in the constant temperature incubator: 10°C, 25°C and 40°C for 48 hours. The concentration values ​​of paclobutrazol / uniconazole were measured at different time points (same as in Example 1). Experimental results such as figure 2As shown, after the whole degradation took place for 48 hours, the degradation rates of paclobutrazol at 10°C, 25°C and 40°C were 20%, 66% and 23%, respectively, and that of uniconazole were 27%, 44% and 20%, respectively. It is proved that laccase still has a certain effect on remediation of pesticide-contaminated soil in an environment of 10°C to 40°C, and the environmental condition of 25°C is more ...

Embodiment 3

[0044] Thoroughly mix the soil to be repaired and add laccase to form 8U / g 土 system, while adding 0.2mg / g of HBT mediator. Then adjust the pH value of the polluted soil sample to the set value with calcium oxide or acetic acid, and use the phosphate concentration as 100mM buffer solution to accurately adjust the pH value to be 3.5, 4.5, 5.5, 6.5 and 7.5 (select the standard pH measurement method, use measured by a pH meter). Keep the soil moisture rate at 15%, all samples are placed in 25 ℃ of constant temperature incubator and cultivate 48h, measure the residual concentration of paclobutrazol / uniconazole in soil respectively at different time points (same as embodiment 1), experimental result is as follows image 3 shown. After the whole degradation took place 48h, the degradation rates of paclobutrazol were 39%, 60%, 35%, 23% and 20% respectively under the conditions of pH=3.5, 4.5, 5.5, 6.5 and 7.5, and the degradation rates of uniconazole were 33%, 33% and 20%, respectiv...

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Abstract

The invention provides a remediation method for pesticide-contaminated soil based on degradation by laccase. The method comprises the following steps: controlling the pH of the soil to be remediated to 3.5-7.5, adding the laccase and mediator HBT, adjusting the moisture content of the soil to 15-60%, and in the environment of 10-40 DEG C, performing illumination by natural light while keeping continuous ventilation for 24-48 h to remediate the pesticide-contaminated soil. The method provided by the invention adopts the laccase to remediate the pesticide-contaminated soil, and has the advantages of high efficiency, environmentally friendliness and no secondary pollution; the property range of soil applicable to the laccase used in the method is wide, the laccase has a good effect when usedin the natural environment, and the catalytic reaction can be performed under milder conditions; the HBT mediator used in the method promotes binding sites of the laccase and target pollutants, maximizes the laccase reaction efficiency and reduces the reaction time of the entire reaction system; therefore, the method has the characteristics of a short remediation period, a good remediation effectand no secondary pollution.

Description

[0001] (1) Technical field [0002] The invention belongs to the technical field of soil remediation polluted by pesticides, and in particular relates to a soil remediation method polluted by pesticides based on laccase degradation. [0003] (2) Background technology [0004] In recent years, the direct application of various pesticides and the indirect entry of industrial wastewater and solid waste into farmland have caused serious challenges to the quality and safety of farmland soil and seriously threatened the safety of agricultural and food production. Studies have shown that farmland soil pollution is divided into organic pollution and inorganic pollution. Organic pollution mainly includes pesticides and organic compounds (such as: NBFRs, PAHs, PAEs, DPs, etc.), and inorganic pollution mainly includes heavy metals, chemical reagents (such as: acids, alkalis, salts, etc.) Wait). Among them, pesticide pollution, as a typical event of farmland soil pollution, has been repor...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/105
Inventor 张安平孙建强陶宁尔吴义华王康
Owner ZHEJIANG UNIV OF TECH
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