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A method for extracting and preparing mannose from dragon fruit stem

A technology for pitaya stems and mannose, applied in chemical instruments and methods, preparation of sugar derivatives, monosaccharides, etc., can solve the problems of low comprehensive utilization of pitaya stems, short dragon fruit industry chain, etc., and achieve improved extraction Rate and utilization rate, raw material ingredient safety, cost reduction effect

Active Publication Date: 2020-06-26
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of this, studying a kind of mannose derived from pitaya stems can not only improve the extraction rate of mannose, reduce the cost of producing mannose and ensure the safety of mannose products, but also solve the problem of low comprehensive utilization of pitaya stems, Problems such as short dragon fruit industry chain can also protect the environment and provide consumers with more choices

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A method for extracting and preparing mannose from dragon fruit stems, comprising the following steps:

[0050] S1. Preprocessing

[0051] Take fresh dragon fruit stems, wash them, peel them, and cut them into pieces; then add a color-protecting aqueous solution for 50 minutes according to the ratio of material to liquid at 1:8, and the color-protecting aqueous solution contains a mass fraction of 0.05% sodium D-ascorbate and 0.3% An aqueous solution of citric acid; finally put it into a blender and stir until it becomes a paste to obtain a dragon fruit stem slurry, which is stored at 4°C.

[0052] S2. Polysaccharide extraction

[0053] S21. Crude polysaccharide mention:

[0054] Adopt ultra-high pressure assisted hot water leaching method to extract, add described pitaya stem slurry in ultra-high pressure extraction equipment, add water according to the ratio of material to liquid 1:15, pressurize and maintain pressure, then release pressure, filter, and obtain The ...

Embodiment 2

[0076] A method for extracting and preparing mannose from dragon fruit stems, comprising the following steps:

[0077] S1. Preprocessing

[0078] Take fresh dragon fruit stems, wash them, peel them, branch them, and cut them into pieces; then add a color-protecting aqueous solution according to the ratio of material to liquid at 1:6 for color protection for 60 minutes, and the color-protecting aqueous solution contains a mass fraction of 0.02% D-sodium ascorbate and an aqueous solution of 0.5% citric acid; finally put it into a blender and stir until it becomes a paste to obtain a dragon fruit stem slurry, which is stored at 4°C.

[0079] S2. Polysaccharide extraction

[0080] S21. Crude polysaccharide mention:

[0081] Use ultra-high pressure assisted hot water extraction method to extract, add the pitaya stem slurry into the ultra-high pressure extraction equipment, add water according to the ratio of material to liquid at 1:20, pressurize and maintain the pressure, then r...

Embodiment 3

[0103] A method for extracting and preparing mannose from dragon fruit stems, comprising the following steps:

[0104] S1. Preprocessing

[0105] Take fresh pitaya stems, wash them, peel them, branch them, and cut them into pieces; then add a color-protecting aqueous solution according to the ratio of material to liquid at 1:10 for color protection for 60 minutes, and the color-protecting aqueous solution contains a mass fraction of 0.1% sodium D-ascorbate and an aqueous solution of 0.1% citric acid; finally put it into a blender and stir until it becomes a paste to obtain a dragon fruit stem slurry, which is stored at 4°C.

[0106] S2. Polysaccharide extraction

[0107] S21. Crude polysaccharide mention:

[0108] Ultra-high pressure assisted hot water extraction method is used for extraction, the dragon fruit stem slurry is added to the ultra-high pressure extraction equipment, water is added according to the ratio of material to liquid at 1:10, the pressure is pressurized ...

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Abstract

The invention provides a method of extracting and preparing mannose from pitaya stems, and belongs to the technical field of mannose preparation. The method includes the steps of pitaya stem preprocessing, polysaccharide extraction, polysaccharide deproteinization, polysaccharide decoloration, polysaccharide small-molecule impurity removal, polysaccharide alcohol precipitation and drying, mannoseoligosaccharide preparation, mannose transformation, high-purity mannose preparation, and the like. According to the method of extracting and preparing the mannose from the pitaya stems, pitaya by-products, namely the pitaya stems serve as a raw material, ultrahigh-pressure-assisted hot water extraction is adopted and combined with a modern enzymatic method to prepare pitaya stem polysaccharides,secondary extraction is conducted, and the extraction rate and utilization rate of crude pitaya stem polysaccharides are significantly increased. By adopting a modern bio-enzyme technology and a membrane separation technology, in combination with an organic solvent replacement crystallization method, a mannose crystal product with the purity of 90% or above is directly prepared simply through one-step crystallization without purification, thus, the technology is simple, and the mannose crystal product is easy to produce.

Description

technical field [0001] The invention relates to the technical field of mannose preparation, in particular to a method for extracting and preparing mannose from dragon fruit stems. Background technique [0002] Mannose (Mannose): It is a monosaccharide and a six-carbon sugar, which is an isomer of glucose and galactose. The most common in nature is D-mannose. Mannose exists in a wide range of forms and is a component of most polysaccharides; at the same time, there are also a large amount of mannose in oligosaccharides in plants and cell walls. Mannose is a widely used functional sugar alcohol. Mannose and its derivatives are often used in medicine, food industry and other industries, and are beneficial to patients with diabetes, obesity, constipation, high cholesterol, cystitis and urethritis. Good adjuvant therapeutic effect. The unique properties of mannose determine that it has unique medicinal uses. The development of mannose products not only plays an important role ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02C12P19/14C12P19/24C07H1/06C07H3/02
CPCC07H1/06C07H3/02C12P19/02C12P19/14C12P19/24
Inventor 唐雅园何雪梅孙健李昌宝辛明李丽盛金凤杨莹李志春郑凤锦李杰民刘国明零东宁
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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